Collagenase, Type II MedChemExpress (MCE)

Product Name	collagenase from clostridium histoly-ticum
Synonyms	Collagenase Collagenase A COLLAGENASE TYPE X collagenase type vii collagenase type I-S clostridiopeptidase A collagenase F. clostridium histolyticum Synonyms Collagenase Collagenase A COLLAGENASE TYPE X collagenase type vii collagenase type I-S clostridiopeptidase A collagenase F. clostridium histolyticum collagenase from achromobacter iophagus collagenase sterile filtered type vii-S Collagenase from Clostridium histolyticum collagenase from clostridium histoly-ticum collagenase crude type ia cell culture*tested Collagenase, Type I, Clostridium histolyticum Collagenase, Type II, Clostridium histolyticum Collagenase, Type III, Clostridium histolyticum Collagenase, Paralithodes camtschatica Hepatopancreas
CAS	9001-12-1
EINECS	232-582-9
Chemical Formula	C60H100N2
Molecular Weight	849.4494
inchi
Package	25 mg;50 mg;100 mg
Price	Email to quote
Descriptions	Collagenase, Type II Collagenase, Type II MedChemExpress (MCE) HY-E70005B 9001-12-1 Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US Descriptions Collagenase, Type II Collagenase, Type II MedChemExpress (MCE) HY-E70005B 9001-12-1 Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US may vary elsewhere. Collagenase, Type II is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type II breaksdown collagens1, 3, 5, 7, 8, 10, fibronectin, gelatin, aggrecann. Type II collagenase is recommended for isolation of bone, heart, liver, thyroid, and salivary gland primary cells.Preparation of storage solution1. Add 1 mL Hank's Balanced Salt Solution (HBSS) with calcium and magnesium directly to 1 g vial of Collagenase.Vortex gently to ensure complete dissolution, and prepare a stock solution of 100 mg/mL (100X stock solution).2. Filter sterilize 100X stock solution using a 0.22 μm filter with a low protein binding filtration unit. Use immediately or dispense into aliquots and store at -20°C to -5°C protected from light.3. Thaw on ice prior to use. Commonly used concentrations for tissue and cell dispersion are 0.5-2.5 mg/mL and for cartilage digestion are 1-2 mg/mL, but the optimal working concentration required needs to be determined based on specific experimental conditions or by referring to the appropriate literature.Dissociate Tissue1. Mince tissue into 3-4 mm pieces with a sterile scalpel or scissors.2. Wash the tissue pieces several times with HBSS containing calcium and magnesium.3. Add sufficient HBSS with calcium and magnesium to submerge tissue. Add collagenase to required working concentration.4. Incubate at 37°C for 4-18 hours. Increased efficiency is obtained using a rocker platform and supplementing the digest with 3 mM CaCl2.5. Disperse cells by passing through a sterile stainless steel or nylon mesh. Remaining tissue fragments may be disaggregated by addition to fresh collagenase solution and further incubation at 37°C.6. Wash dispersed cells several times by centrifugation in HBSS w/o collagenase.7. Resuspend cell pellet, after the final wash step, in culture medium. Determine viable cell density using a Automated Cell Counter (alternate automated or manual methods may be used).8. Seed cells into culture vessels containing appropriate media.Organ Perfusion1. Add collagenase to prewarmed (37°C) HBSS with calcium and magnesium. Addition of 3 mM CaCl2 increases the efficiency of dissociation.2. Perfuse organ at preoptimized rate for the particular organ.3. Dispersed cells and tissue fragments are separated from larger pieces by passing the perfusate through a sterile stainless steel or nylon mesh. Remaining tissue fragments may be disaggregated by addition to fresh collagenase solution and further incubation at 37°C.4. The steps are the same as for tissue isolation 6-8. \| \| \| \| \| \| \| \| \| \| [1]. Hamzeh Alipour, et al. Therapeutic applications of collagenase (metalloproteases): A review. Asian Pac J Trop Biomed. 2016, 6, 11.
Supplier Website	http://www.medchemexpress.com/collagenase-type-ii.html
Last Update	2025-10-14 16:03:33

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