L-NAME hydrochlorideL-NAME hydrochloride
MedChemExpress (MCE)
HY-18729A
51298-62-5
NG-Nitroarginine methyl ester hydrochloride
99.70%
-20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months
-20°C, 1 month (sealed storage, away from moisture)
Room temperature in continental US
may vary elsewhere.
L-NAME hydrochloride inhibits NOS with an IC50 of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC50 value of 1.4 μM.
L-arginine analogues are widely used inhibitors of nitric oxide synthase (NOS) activity, with Nw-nitro-L-arginine methyl ester (L-NAME) being at the head[2]. Freshly dissolved L-NAME is a 50 fold less potent inhibitor of purified brain NOS (mean IC50= 70 μM) than L-NOARG (IC50= 1.4 μM), but the apparent inhibitory potency of L-NAME approached that of L-NOARG upon prolonged incubation at neutral or alkaline pH. HPLC analyses reveal that NOS inhibition by L-NAME closely correlated with hydrolysis of the drug to L-NOARG[1].
L-NAME hydrochloride can be used to induce hypertension models[6]. .f12{ font-size: 12px
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} Induction of hypertension Model[6] Background L-NAME hydrochloride decreases nitric oxide (NO) release with an inhibition competence in endothelial nitric oxide synthase (eNOS) in animals. Specific Mmodeling Methods Mice: Swiss Webster • male • 6-week-old Administration: 400 mg/kg • ip • once daily for 7 days Note Modeling Indicators Body quality changes: Induced hypertension with body weight loss and high blood pressure. Correlated Product(s): / Opposite Product(s): / 2. Induction of preeclampsia[8] Background L-NAME induces preeclampsia by reducing the production of nitric oxide through inhibiting nitric oxide synthase, triggering a series of changes such as vasoconstriction, placental dysfunction, inflammatory response, and anti-angiogenesis. Specific Mmodeling Methods Mice: CBA x C57BL/6 pregnant female mice • 6-16 weeks oldAdministration: subcutaneous injection • 50 mg/kg/day • E7.5 to E17.5 Modeling Indicators Elevated blood pressure, impaired fetal and placental growth, and increased circulating endothelin-1 (vasoconstrictor), soluble fms-like tyrosine kinase-1 (anti-angiogenic factor), and C-reactive protein (inflammatory marker). Correlated Product(s): / Opposite Product(s): /
IC50: 70 μM (NOS)[1] Cellular Effect Cell Line Type Value Description References
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[1]. Pfeiffer S, et al. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement forbioactivation to the free acid, NG-nitro-L-arginine. Br J Pharmacol. 1996 Jul
118(6):1433-40. [Content Brief]
[2]. Kopincová J, et al. L-NAME in the cardiovascular system - nitric oxide synthase activator? Pharmacol Rep. 2012
64(3):511-20. [Content Brief]
[3]. Lo HC, et al. The Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester Diminishes the Immunomodulatory Effects of Parental Arginine in Rats with Subacute Peritonitis. PLoSOne. 2016 Mar 23
11(3):e0151973. [Content Brief]
[4]. Luo H, et al. Effect of nitric oxide synthase inhibitor L-NAME on fear extinction in rats: a task-dependent effect. Neurosci Lett. 2014 Jun 20
572:13-8. [Content Brief]
[5]. Ocsan RJ, et al. Chronic NG-nitro-l-arginine methyl ester (L-NAME) administration in C57BL/6J mice induces a sustained decrease in c-kit positive cells during development of cardiac hypertrophy. J Physiol Pharmacol. 2013 Dec
64(6):727-36. [Content Brief]
[6]. V A Peotta, et al. Cardiovascular neural reflexes in L-NAME-induced hypertension in mice. Hypertension. 2001, 38, 3. [Content Brief]
[7]. Xiaofei Li, et al. Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the l-NAME-induced hypertensive rat model. Food Funct. 2016, 7, 5. [Content Brief]
[8]. de Alwis N, et al. The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health. Life Sci Alliance. 2022 Aug 5
5(12):e202201517. [Content Brief]
