Protease K MedChemExpress (MCE)

Product Name	proteinase K for tritirachium album
Synonyms	3.4.21.14 Protease K EC 3.4.21.14 PROTEINASE K EC 3.4.21.64 proteinase K crude Recombinant Proteinase K Synonyms 3.4.21.14 Protease K EC 3.4.21.14 PROTEINASE K EC 3.4.21.64 proteinase K crude Recombinant Proteinase K PROTEINASE K, LYOPHILIZED PROTEINASE K, IMMOBILIZED Tritirachium album proteinase K proteinase K aseptically filled proteinase K for tritirachium album proteinase K from tritrachium album ProteinaseK,PowderAndSolutionAvailable ProteinaseK,fromTritirachiumalbumLimber PROTEINASEK,LYOPHILIZEDPOWDER,BIOTECHGRADE Proteinase K for tritirachium album serine. proteinase K from tritirachium album*molecular bi Proteinase K from Tritirachium album limber, Recombinant Proteinase K from Tritirachium album [EC 3.4.21.64] from Tritirachium album
CAS	39450-01-6;102925-54-2
EINECS	254-457-8
Chemical Formula	C29H27N2O12P
Molecular Weight	626.504641
inchi
Package	5 mg;10 mg;25 mg;50 mg;100 mg;500 mg
Price	Email to quote
Descriptions	Proteinase K Proteinase K MedChemExpress (MCE) HY-108717 39450-01-6 Protease K Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US Descriptions Proteinase K Proteinase K MedChemExpress (MCE) HY-108717 39450-01-6 Protease K Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US may vary elsewhere. Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection. Proteinase K enzyme can be used to break amid bond between MTX and BSA and also amidic bonds in BSA structure[2]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Proteinase K with superior protein-degrading property, can be used to detect the SARS-COV2 viral component directly from inoculated viral transport medium (VTM) without RNA extraction[3]. Protocol of rRTPCR detection of SARS-CoV2[3]: 1.Separate VTM of each SARS-COV2 samples into three EP tubes (45 渭L per tube) 2.Add 2.5 渭L (20 mg/mL concentrated) proteinase K (PK), making a final concentration of PK ~1 mg/ml VTM 3.Incubate at 25掳C for 5 min 4.Incubate at 60掳C for 15 min 5.Incubate at 98掳C for 10 min, and store samples at 4掳C 6.Use 5 渭L of extracted RNA as template to execute rRTPCR assay 7.Exert rRTPCR detection by BIORAD CFX96? Real-Time Thermocycler, with a positive standard of threshold cycle (Ct) values less than 40. Proteinase K improves the homogenization effects and positive rates of sputum samples, and increases nucleic acid concentration extracted from it[4]. Protocol of nucleic acid extraction of influenza A virus (IAV)[4]: 1.Separate specimen into three EP tubes (500 渭L per tube) 2.Add 500 渭L 0.4 mg/mL Proteinase K buffer, vortex for 20 seconds 3.Incubate at 55掳C for 25 min, vortex 5 seconds every 5 min 4.Extract RNA by using the Automated Nucleic Acid Extraction System through a paramagnetic beads method (Zhijiang Biotechnology Co., Ltd., Shanghai, China) with 200 渭L samples 5.Evaluate A260/A280 ratio of pure RNA products, with a standard ranged from 1.8 to 2.0. \| \| \| \| \| \| \| \| \| \| [1]. W Ebeling, et al. Proteinase K From Tritirachium Album Limber. Eur J Biochem . 1974 Aug 15 47(1):91-7. [Content Brief] [2]. Nosrati H, et al. Multifunctional nanoparticles from albumin for stimuli-responsive efficient dual drug delivery. Bioorg Chem. 2019 Jul 88:102959. [Content Brief] [3]. Shukla A, et al. Vitality of Proteinase K in rRTPCR Detection of SARS-CoV2 Bypassing RNA Extraction. Front Cell Infect Microbiol. 2021 Nov 3 11:717068.聽 [Content Brief] [4]. Yu F, et al. Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum. Front Med (Lausanne). 2018 Mar 2 5:56. [Content Brief]
Supplier Website	http://www.medchemexpress.com/protease-k.html
Last Update	2025-10-14 16:03:33

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