Hoechst 33342 MedChemExpress (MCE)

Product Name	2'-(4'-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bis-1H-benzimidazole trihydrochloride trihydrate
Synonyms	Ho 342 Hoe 33342 Bisbenzimide Hoechst 33342 bis-benzimide (ethoxyphenyl form) CELLSTAIN- HOECHST 33342 SOLUTION 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Synonyms Ho 342 Hoe 33342 Bisbenzimide Hoechst 33342 bis-benzimide (ethoxyphenyl form) CELLSTAIN- HOECHST 33342 SOLUTION 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole 2,5'-Bibenzimidazole, 2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl) 2,5'-Bibenzimidazole, 2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)- 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-5'-bi-1h-benzimidazole 2,5-Bi-1H-benzimidazole, 2-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)- 2'-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzimidazole 2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)- (9CI) 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride 2'-(4'-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bis-1H-benzimidazole trihydrochloride trihydrate bisBenzimide, HOE 33342, Hoechst 33342, 2μ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole trihydrochloride
CAS	23491-52-3
EINECS	245-691-1
Chemical Formula	C27H28N6O
Molecular Weight	452.55
inchi	InChI=1/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
Package	10 mg;25 mg;50 mg;100 mg
Price	Email to quote
Descriptions	Hoechst 33342 Hoechst 33342 MedChemExpress (MCE) HY-15559 23491-52-3 bisBenzimide H 33342 Descriptions Hoechst 33342 Hoechst 33342 MedChemExpress (MCE) HY-15559 23491-52-3 bisBenzimide H 33342 HOE 33342 99.94% 4°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. Hoechst 33342 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. General Protocol Preparation of Hoechst working solution 1.1 Preparation of the stock solution Dissolve 10 mg of in 5 mL DMSO Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Hoechst working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working solution according to the actual situation. 1.Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. Precautions 1. Please adjust the concentration of Hoechst working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate. Labeling Nuclear DNA with Hoechst 33342[1]Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+.Step 5, Mount the coverslips.Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1]. Dye reagent[1]DNA Stain[1] Cellular Effect Cell Line Type Value Description References 452 356 \| \| \| \| \| \| \| \| \| \| [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1 2011(1):pdb.prot5557. [Content Brief]*
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Last Update	2025-10-14 16:03:33

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