Dihydroethidium MedChemExpress (MCE)

Product Name	dihydroethidium
Synonyms	PD-MY 003 PD-MY 003 HYDROETHIDINE dihydroethidium (DIHYDROETHIDIUM BROMIDE) DIHYDROETHIDIUM, FOR FLUORESCENCE* 5-ethyl-6-phenyl-5,6-dihydrophenanthridine-3,8-diaMine Synonyms PD-MY 003 PD-MY 003 HYDROETHIDINE dihydroethidium (DIHYDROETHIDIUM BROMIDE) DIHYDROETHIDIUM, FOR FLUORESCENCE* 5-ethyl-6-phenyl-5,6-dihydrophenanthridine-3,8-diaMine 3,8-Phenanthridinediamine,5-ethyl-5,6-dihydro-6-phenyl- 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine Hydroethidine
CAS	104821-25-2
EINECS
Chemical Formula	C21H21N3
Molecular Weight	315.41
inchi	InChI=1/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
Package	1 mg;5 mg;10 mg;25 mg;50 mg;100 mg
Price	Email to quote
Descriptions	Dihydroethidium Dihydroethidium MedChemExpress (MCE) HY-D0079 104821-25-2 Hydroethidine Descriptions Dihydroethidium Dihydroethidium MedChemExpress (MCE) HY-D0079 104821-25-2 Hydroethidine PD-MY 003 98.71% 4°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation. General ProtocolPreparation of Dihydroethidium working solution1.1 Preparation of the stock solutionDissolve 1 mg of Dihydroethidium in 0.31 mL of DMSO to obtain 10 mM of Dihydroethidium.Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.1.2 Preparation of Dihydroethidium working solutionDilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of Dihydroethidium working solution.Note: Please adjust the concentration of Dihydroethidium working solution according to the actual situation.Cell staining2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.2.2 Add 1 mL of Dihydroethidium working solution, and then incubate at room temperature for 30 minutes.2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.2.4 Wash twice with PBS, 5 minutes each time.2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.Storage-20°C, 1 yearProtect from lightPrecautions1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.2. Please adjust the concentration of Dihydroethidium working solution according to the actual situation.3. This product is for R&D use only, not for drug, household, or other uses.4. For your safety and health, please wear a lab coat and disposable gloves to operate. 616 518 \| \| \| \| \| \| \| \| \| \| [1]. Zielonka J, et al. Global profiling of reactive oxygen and nitrogen species in biological systems: high-throughput real-time analyses. J Biol Chem. 2012 Jan 27 287(5):2984-95. [Content Brief]*
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Last Update	2025-10-14 16:03:33

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