Rhod-2 AM MedChemExpress (MCE)

Product Name	RHOD-2, AM
Synonyms	RHOD 2 RHOD-2, AM XANTHYLIUM Xanthylium Rhod 2 RHIZOMADIBOTRYISEXTRACT 9-[4-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-3,6-bis(dimethylamino)xanthylium Bromide
CAS	145037-81-6
EINECS
Chemical Formula	C52H59BrN4O19
Molecular Weight	1123.94
inchi
Package	50 μg;1 mg
Price	Email to quote
Descriptions	Rhod-2 AM Rhod-2 AM MedChemExpress (MCE) HY-D0989 145037-81-6 96.30% -20°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months Descriptions Rhod-2 AM Rhod-2 AM MedChemExpress (MCE) HY-D0989 145037-81-6 96.30% -20°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months -20°C, 1 month (sealed storage, away from moisture and light) Room temperature in continental US may vary elsewhere. Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm. 1.Preparation of Rhod-2 AM working solution1.1Preparation of the stock solutionDissolve Rhod-2 AM in DMSO to obtain 5 mM of stock solution.1.2Preparation of Rhod-2 AM working solutionDilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of working solution.Note: Please adjust the concentration of Rhod-2 AM working solution according to the actual situation.2.Cell staining (6-well plate)2.1Suspension cellsa.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.d.Wash twice with PBS, 5 minutes each time.e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.2.2 Adherent cellsa.Culture adherent cells on sterile coverslips.b.Remove the coverslip from the medium and aspirate excess medium.c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.d.Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1]. 578 549 \| \| \| \| \| \| \| \| \| \| [1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec 84(23):12226-35. [Content Brief] [2]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec 84(23):12226-35. [Content Brief]
Supplier Website	http://www.medchemexpress.com/Rhod-2_AM.html
Last Update	2025-10-14 16:03:33

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