5-Hydroxymethylcytosine-¹³C,d₂  (Synonyms: 5hmC-¹³C,d₂)

5-Hydroxymethylcytosine-¹³C,d₂ is the ¹³C and deuterium labeled 5-Hydroxymethylcytosine (HY-W018324). 5-Hydroxymethylcytosine (5hmC) is an oxidized forms of 5-methylcytosine (5mC) in mammalian DNA. 5-Hydroxymethylcytosine is produced from 5mC in an enzymatic pathway involving three 5mC oxidases, Ten-eleven translocation (TET)1, TET2, and TET3. The conversion of 5mC into 5hmC can be the first step in a pathway leading towards DNA demethylation. 5-Hydroxymethylcytosine is associated with gene transcription and frequently used as a mark to investigate dynamic DNA methylation conversion during mammalian development. 5-Hydroxymethylcytosine can be used for the study of non-small cell lung cancer (NSCLC), neurodegenerative diseases (Alzheimer’s, Parkinson’s) and hematological malignancies (acute myeloid leukemia, myelodysplastic syndromes)^[1][2][3][4].

氢、碳和其他元素的稳定重同位素已被纳入药物分子中，主要作为药物开发过程中定量的示踪剂。氘化引起了人们的关注，因为它可能影响药物的药代动力学和代谢谱。5-Hydroxymethylcytosine (5hmC) 是一种稳定的表观遗传修饰，由十-十一转位 (TET) 酶 (TET1、TET2、TET3) 氧化5-甲基胞嘧啶 (5mC) 生成。5-Hydroxymethylcytosine 既是主动DNA去甲基化的中间体，又是独立的基因表达调控因子，富集于 CpG 岛、活跃/双价启动子和转录增强子，促进开放染色质形成并调节转录因子结合。5-Hydroxymethylcytosine 在生物过程中发挥关键作用：维持胚胎干细胞 (ESC) 多能性、驱动神经发育和抑制肿瘤进展^[1][2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

144.13

C₄¹³CH₅D₂N₃O₂

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-220.  [Content Brief]

  [2]. Zheng K, et al. 5-Hydroxymethylcytosine: Far Beyond the Intermediate of DNA Demethylation. Int J Mol Sci. 2024 Nov 2;25(21):11780.  [Content Brief]

  [3]. Pfeifer GP, et al. 5-hydroxymethylcytosine and its potential roles in development and cancer. Epigenetics Chromatin. 2013 May 1;6(1):10.  [Content Brief]

  [4]. Cui XL, et al. A human tissue map of 5-hydroxymethylcytosines exhibits tissue specificity through gene and enhancer modulation. Nat Commun. 2020 Dec 2;11(1):6161.  [Content Brief]