8-pCPT-2′-O-Me-cAMP  (Synonyms: 8-CPT-2'-O-Me-cAMP)

8-pCPT-2′-O-Me-cAMP (8-CPT-2'-O-Me-cAMP), an analog of cAMP, is an activator of exchange proteins activated by cAMP (Epac). 8-pCPT-2′-O-Me-cAMP activates Epac1 (EC₅₀ = 2.2 μM), but not PKA (EC₅₀ >10 μM). 8-pCPT-2′-O-Me-cAMP stimulates Epac-mediated Ca²⁺ release in pancreatic β-cells in vitro. 8-pCPT-2′-O-Me-cAMP is a Rap1 activator. 8-pCPT-2′-O-Me-cAMP enhances the retinal pigment epithelium barrier against the pathological choroidal endothelial cell invasion that occurs in macular degeneration^[1][2][3].

Rap1^[1]

8-CPT-2'-O-Me-cAMP (250 µM; 1 h) 诱导 Rap1 活化并增强视网膜色素上皮细胞中连接蛋白和皮质 F-肌动蛋白的募集^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

8-CPT-2'-O-Me-cAMP (2.05 µM; 1 µL; intravitreal injection) 可抑制激光损伤模型中脉络膜新生血管病变的发展^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

485.84

C₁₇H₁₇ClN₅O₆PS

510774-50-2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wittchen ES, et al. Rap1 GTPase activation and barrier enhancement in rpe inhibits choroidal neovascularization in vivo. PLoS One. 2013 Sep 10;8(9):e73070.  [Content Brief]

  [2]. Enserink JM, Christensen AE, de Rooij J, van Triest M, Schwede F, Genieser HG, Døskeland SO, Blank JL, Bos JL. A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK. Nat Cell Biol. 2002 Nov;4(11):901-6.  [Content Brief]

  [3]. Kang G, Joseph JW, Chepurny OG, Monaco M, Wheeler MB, Bos JL, Schwede F, Genieser HG, Holz GG. Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic beta-cells. J Biol Chem. 2003 Mar 7;278.  [Content Brief]