Acetoacetyl-CoA sodium hydrate  (Synonyms: 乙酰乙酰辅酶A钠盐水合物; 3-Oxobutanoyl-coenzyme A sodium hydrate)

Acetoacetyl CoA sodium hydrate is the precursor of HMG-CoA in the mevalonate pathway. Acetoacetyl-CoA thiolase catalyzes the reaction to form acetoacetyl-CoA sodium hydrate from two acetyl-CoA molecules. Acetoacetyl CoA sodium hydrate is essential for cholesterol biosynthesis. Acetoacetyl-CoA sodium hydrate is also a intermediate in the biological breakdown and synthesis of fatty acids^[1][2][3].

纯化的重组 Msed_0389 和 Msed_1423 催化 NAD 依赖的 (S)-3-羟基丁酰辅酶 A 氧化为 Acetoacetyl-CoA ，并且对其底物具有高度特异性 (Km 分别为 2.6 μM 和 5 μM)^[2]。
同源双功能蛋白是唯一能够在自养脱硫球菌目 (Ignicoccus hospitalis) 和嗜热菌目 (Pyrobaculum neutrophilus) 中将巴豆酰辅酶 A 转化为 Acetoacetyl-CoA 的酶，它们利用二羧酸/4-羟基丁酸循环进行 CO2 固定^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

固体

White to off-white

乙酰乙酰辅酶A钠盐水合物

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Miziorko HM. Enzymes of the mevalonate pathway of isoprenoid biosynthesis. Arch Biochem Biophys. 2011 Jan 15;505(2):131-43.  [Content Brief]

  [2]. Liu L, et al. Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula. Front Microbiol. 2020 Mar 11;11:354.  [Content Brief]

  [3]. Feodor Lynen, et al. Enzymes of fatty acid metabolism. Biochimica et Biophysica Acta. Volume 12, Issues 1-2, October 1953, Pages 299-314.