Acridine Orange hydrochloride (solution) 

Acridine Orange hydrochloride (solution) is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm) ^[1][2][3].
Solvent and Concentration: Sterile water: 10 mg/mL

指南 (以下是我们推荐的方案。此方案仅提供指南，应根据您的具体需求进行修改)。^[4].
1. 用 Acridine Orange hydrochloride 染色细胞 (1 μM; 20 分钟; 37 °C)。
2. 用 PBS 洗涤细胞。
3. 通过共聚焦激光扫描显微镜 (FV3000，Olympus) 观察细胞。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Acridine Orange hydrochloride (0.1 mg/kg，静脉注射，狗) 在 30 天内没有表现出临床毒性迹象，血液中也没有发现异常^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

301.82

C₁₇H₂₀ClN₃

65-61-2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.  [Content Brief]

  [2]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.  [Content Brief]

  [3]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.  [Content Brief]

  [4]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.  [Content Brief]