2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Aldehyde Dehydrogenase (ALDH)
  4. AlDeSense AM

AlDeSense AM 是一种可透过细胞膜的 ALDH1A1 选择性荧光报告剂。AlDeSense AM 可被 ALDH1A1 氧化，从而消除光诱导电子转移淬灭作用并增强荧光信号。AlDeSense AM 可用于检测具有癌症干细胞特性的细胞，以及在细胞培养体系和动物模型中监测癌症干细胞的可塑性。AlDeSense AM 可用于研究与癌症干细胞相关的癌症，包括慢性髓系白血病、黑色素瘤、乳腺癌。

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AlDeSense AM

AlDeSense AM Chemical Structure

CAS No. : 2243148-62-9

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AlDeSense AM 相关抗体

Top Publications Citing Use of Products

查看 Aldehyde Dehydrogenase (ALDH) 亚型特异性产品:

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ALDH1 ALDH2 ALDH3

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

AlDeSense AM is a cell-permeable ALDH1A1-selective fluorescent reporter. AlDeSense AM is oxidized by ALDH1A1, which eliminates photoinduced electron transfer quenching and enhances the fluorescent signal. AlDeSense AM can be used to detect cells with cancer stem cell properties, as well as to monitor the plasticity of cancer stem cells in cell culture systems and animal models. AlDeSense AM is applicable to the study of cancers associated with cancer stem cells, including chronic myeloid leukemia, melanoma, and breast cancer[1].

体外研究
(In Vitro)

AlDeSense AM (1.5-2 μM; 30 min) 可选择性标记具有活性 ALDH1A1 的人慢性髓系白血病 K562 细胞，这些细胞对应 CD34+/CD38−/CD133+ 白血病干细胞群[1]。
AlDeSense AM (2 μM; 1 h) 可检测 MDA-MB-231 乳腺癌乳腺球 (富集癌症干细胞) 中升高的 ALDH1A1 活性，且随着乳腺球在 36 h 内分化，荧光信号会下降[1]。
AlDeSense AM (2 μM; 1 h) 在富集 CSC 的 B16F0 e-CSC 中的荧光信号比非 CSC 的 B16F0 细胞高 11.3 倍，证实 e-CSC 中 ALDH1A1 活性升高[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

AlDeSense AM 相关抗体:

Immunofluorescence[1]

Cell Line: K562 human chronic myeloid leukemia cells
Concentration: 1.5 μM (flow cytometry staining); 2 μM (confocal imaging staining)
Incubation Time: 30 min at room temperature (confocal imaging staining)
Result: Identified a population of brightly fluorescent cells corresponding to the CD34+/CD38-/CD133+ leukemic stem cell profile.
Showed a small population of highly fluorescent cells via confocal imaging.
Completely abolished the fluorescence signal after ALDH1A1 siRNA knockdown.

Immunofluorescence[1]

Cell Line: MDA-MB-231 breast cancer mammospheres (CSC-enriched) and differentiating mammospheres
Concentration: 2 μM
Incubation Time: 1 h at room temperature (at 1, 6, 12, 24, 36 h time points during differentiation)
Result: Exhibited 3-fold brighter fluorescence in freshly isolated mammospheres compared to control-stained mammospheres.
Gradually decreased fluorescence signal over 36 h of differentiation.

Immunofluorescence[1]

Cell Line: B16F0 murine melanoma e-CSCs (CSC-enriched via spiral-patterned hydrogel culture) and non-CSCs (standard culture)
Concentration: 2 μM
Incubation Time: 1 h at room temperature
Result: Showed 11.3-fold brighter fluorescence in e-CSCs than in non-CSCs.
Showed 9.0-fold brighter fluorescence in e-CSCs than in e-CSCs stained with control reagent.
Colocalized with the CD271 melanoma stem cell marker in e-CSCs.
体内研究
(In Vivo)

AlDeSense AM (15 μM; 肺灌注; 单次给药) 可在 C57BL/6J 小鼠中选择性检测上皮性癌症干细胞 (e-CSC) 来源的肺转移灶中升高的 ALDH1A1 活性，产生的荧光信号显著高于非癌症干细胞来源的转移灶[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (female, 6 to 8 weeks old)[1]
Dosage: 15 μM
Administration: lung perfusion; single dose
Result: Produced a mean fluorescence signal of ~1×1011 a.u.
in lungs with e-CSC metastases.
Showed a significantly higher mean fluorescence signal (~1×1011 a.u.) than the ~7×1010 a.u.
measured in lungs with non-CSC metastases.
Demonstrated significantly higher fluorescence in e-CSC metastases than e-CSC metastases treated with Ctrl-AlDeSense AM.
分子量

438.38

Formula

C24H16F2O6
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

AlDeSense AM 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AlDeSense AM2243148-62-9Fluorescent DyeAldehyde Dehydrogenase (ALDH)melanomabreast cancerCD34+/CD38-/CD133+ leukemic stem cell populationC57BL/6J micechronic myeloid leukemiaMDA-MB-231 breast cancer mammospherescancer stem cellsB16F0 e-CSCsK562 human chronic myeloid leukemia cellsALDH1A1Inhibitorinhibitorinhibit

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