c-Fos 抗体

c-Fos Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to c-Fos.

c-Fos：Fos 基因家族由 4 个成员组成：FOS、FOSB、FOSL1 和 FOSL2。这些基因编码的亮氨酸拉链蛋白可以与 JUN 家族的蛋白二聚化，从而形成转录因子复合物 AP-1。因此，FOS 蛋白被认为是细胞增殖、分化和转化的调节因子。在某些情况下，FOS 基因的表达也与凋亡细胞死亡有关。[由 RefSeq 提供，2008 年 7 月]

Free

P01100

Predicted band size: 41 kDa; Observed band size: 58 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102634

Neuroscience

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; ICC/IF: 1:50-1:200; ELISA: 1:10000

• 
  Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg) using c-Fos Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using c-Fos Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using c-Fos Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80616, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Fos.AA range:331-380