Caveolin-1 抗体 (YA549)

Caveolin-1 Antibody (YA549) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caveolin-1.

Caveolin-1 蛋白作为小凹膜内的支架蛋白发挥着至关重要的作用，与 CAV2 形成稳定的异寡聚复合物，靶向脂筏并驱动小凹的形成。该蛋白参与介导 CAVIN 蛋白 (CAVIN1/2/3/4) 向小凹的募集，突出了其在细胞信号传导中的复杂作用。除了膜组织之外，Caveolin-1 还直接与 G 蛋白 α 亚基相互作用，影响其活性，并参与 T 细胞受体 (TCR) 介导的 T 细胞激活所需的共刺激信号。此外，它与 DPP4 的结合以 T 细胞受体/CD3 依赖性方式诱导 T 细胞增殖和 NF-κ-B 激活。Caveolin-1 进一步将 CTNNB1 招募到小凹膜，可能通过 Wnt 途径调节 CTNNB1 介导的信号传导。值得注意的是，它通过促进 TGFBR1 从膜筏的内化和随后的降解来负调节 TGFB1 介导的 SMAD2/3 激活。通过与 BMX、BTK、CTNNB1、CDH1、GLIPR2、JUP、NOSTRIN、SNAP25、STX1A、SLC7A9、PACSIN2、EHD2、CAVIN3 和 ABCG1 等多种蛋白质的多种相互作用，Caveolin-1 协调多种细胞功能，有助于膜动力学、信号传导和胆固醇稳态的动态调节。

Free

Q03135

Predicted band size: 20 kDa; Observed band size: 20 kDa

Protein A affinity purified.

Golgi apparatus membrane, Cell membrane, Membrane.

Non-conjugated

Unmodified

AB_3102284

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:5,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) using Caveolin-1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Caveolin-1.AA range:129-178.