IL17A 抗体

目录号: HY-P81114: Data Sheet SDS COA 抗体使用指南
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IL17A Antibody 是一个兔来源、无偶联标记、抗 IL17A 的 IgG 多克隆抗体。

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WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

产品详情
验证图片
背景信息
产品资料

描述

IL17A Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL17A.

宿主

Rabbit

克隆性

Polyclonal

分子量

Predicted band size: 15 kDa;

Observed band size: 22 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Mouse, Rat,

蛋白数据库

Q62386

基因 ID

16171 [NCBI]

免疫原

KLH conjugated synthetic peptide derived from mouse IL-17: 85-150/150

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-2000
ELISA ELISA: 酶联免疫吸附试验	1:5000-10000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:100-500
IHC-F IHC-F: 冰冻切片样本的免疫组织化学	1:100-500
ICC/IF ICC/IF: 细胞免疫荧光	1:100-500

敏感性	Endogenous	纯度	affinity purified
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

IHC

Immunohistochemical analysis of paraffin-embedded human Mouse Lung tissue using IL17A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81114, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Mouse Lung tissue using IL17A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81114, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Mouse Lung tissue using IL17A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81114, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human ‌Rat spleen‌‌ tissue using IL17A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81114, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Rat Ovary tissue using IL17A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81114, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Rat Ovary tissue using IL17A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81114, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

背景

功能：IL-17 是先天性和适应性免疫系统的效应细胞因子，参与抗菌宿主防御和组织完整性的维持 (PubMed:18025225, PubMed:19144317, PubMed:26431948)。它通过 IL-17RA-IL-17RC 异二聚体受体复合物传递信号，触发 IL-17RA 和 IL-17RC 链与 TRAF3IP2 接头蛋白的同型相互作用。这导致下游 TRAF6 介导的 NF-κB 和 MAP 激酶通路激活，最终导致细胞因子、趋化因子、抗菌肽和基质金属蛋白酶的转录激活，并可能引发强烈的免疫炎症反应 (PubMed:16200068, PubMed:17911633, PubMed:19144317, PubMed:26431948)。在连接 T 细胞介导的适应性免疫和急性炎症反应以清除细胞外细菌和真菌方面发挥着重要作用。作为辅助性 T 细胞 17 (Th17) 的标志性效应细胞因子，它主要诱导中性粒细胞活化并募集至感染和炎症部位 (PubMed:18025225)。在气道上皮中，它通过诱导 CXCL1 和 CXCL5 趋化因子介导中性粒细胞趋化 (PubMed:18025225, PubMed:27923703)。在次级淋巴器官中，它通过调节 B 细胞对 CXCL12 和 CXCL13 的趋化反应，促进生发中心的形成，增强 B 细胞在生发中心内的滞留，提高 B 细胞体细胞高频突变率，并促进 B 细胞向浆细胞分化 (PubMed:18157131)。 γδT 细胞亚群的效应细胞因子，作为 IL-1β、TLR2 和 IL-23A-IL-12β下游炎症通路的一部分，促进中性粒细胞募集，从而有效清除细菌 (PubMed:17372004, PubMed:20364087, PubMed:28709803)。固有免疫细胞 (包括不变自然杀伤细胞 (iNKT) 和 3 型固有淋巴细胞) 的效应细胞因子，介导初始中性粒细胞炎症 (PubMed:17470641, PubMed:23255360)。参与维持体内平衡和病原体感染期间上皮屏障的完整性。在急性损伤时，通过调节 OCLN 定位和紧密连接的生物合成，在上皮屏障形成中发挥直接作用 (PubMed:26431948)。作为共生菌诱导的黏膜免疫反应的一部分，该蛋白通过促进中性粒细胞募集和抗菌肽释放，增强宿主抵抗致病菌和真菌感染的能力 (PubMed:28709803)。它与 IL-17F 协同作用，介导黏膜上皮细胞产生抗菌β-防御素 DEFB1、DEFB103A 和 DEFB104A，从而限制微生物穿过上皮屏障 (PubMed:19144317)。该蛋白还通过多种机制参与抗病毒宿主防御 (PubMed:21946434, PubMed:26735852, PubMed:27795421)。此外，它还通过促进 T 细胞的细胞毒性来增强对西尼罗河病毒的免疫力 (PubMed:27795421)。可能通过增强肺部 B 细胞募集和免疫反应，在甲型流感病毒 (H5N1) 感染中发挥有益作用 (PubMed:21946434)。通过促进 B-1a B 细胞分化，在宿主第一道防线产生病毒特异性 IgM 抗体，从而有助于清除甲型流感病毒 (H1N1)(PubMed:26735852)。

亚细胞定位：分泌物

表达水平：
组织特异性：Th17 细胞谱系表达 (蛋白水平) 。其表达模式反映了分化状态，在完全分化的 Th17 细胞中，IL17A-IL17F 异二聚体的产生水平高于 IL17A-IL17A 和 IL17F-IL17F 二聚体 (PubMed:16990136, PubMed:18025225) 。固有淋巴细胞表达 (蛋白水平) (PubMed:23255360, PubMed:28709803) 。γδT 细胞亚群表达 (蛋白水平) (PubMed:17372004, PubMed:20364087, PubMed:26431948, PubMed:28709803) 。iNKT 细胞表达 (蛋白水平) (PubMed:17470641) 。

诱导：在 IL-6 和 TGF-β1 存在的情况下，抗原受体结合诱导 CD4 阳性 T 细胞向 Th17 效应细胞分化 (PubMed:16200068, PubMed:16990136, PubMed:18025225) 。IL-23A-IL-12B、IL-1B 和 TNF 上调该过程，而 IFN-γ和 IL-4 抑制该过程 (PubMed:16200068, PubMed:18025225) 。在多种免疫细胞中，促炎细胞因子可上调该基因的表达，以响应微生物感染：真菌感染可诱导固有淋巴细胞表达该基因；乳杆菌感染可诱导 Vδ4 阳性γδT 细胞表达该基因；金黄色葡萄球菌感染可诱导 Vδ5 阳性γδT 细胞表达该基因；大肠杆菌感染可诱导 Vδ1 阳性γδT 细胞表达该基因 (PubMed:17372004, PubMed:20364087, PubMed:23255360, PubMed:28709803) 。急性损伤可诱导肠固有层γδT 细胞表达该基因 (PubMed:26431948) 。CD1D 刺激可诱导 KLRB1/NK1.1 阴性 iNKT 细胞亚群表达该基因 (PubMed:17470641) 。

亚基：同源二聚体 (PubMed：18025225)。

RRID

AB_3103217

反应种属数据库

Entrez Gene: 16171 Human ; 16171 Mouse

SwissProt: Q62386 Mouse ; Q61453 Rat ;

中文名

白介素-17抗体

同用名

Interleukin-17A; CTLA-8; CTLA 8; CTLA8; Cytotoxic T lymphocyte associated antigen 8; Cytotoxic T lymphocyte associated serine esterase 8; IL 17A; IL-17A; IL-17; Interleukin 17; Interleukin-17; Interleukin 17A; Interleukin17; Interleukin17A; IL17_HUMAN; IL17_MOUSE.

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (192 KB)

抗体使用指南 (1083 KB)

IL17A Antibody 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IL17A 抗体

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IL17A 抗体

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