Integrin beta 1 抗体 (YA337)

Integrin beta 1 Antibody (YA337) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin beta 1.

Integrin beta 1 是一种多功能细胞表面受体，可与多种 alpha 亚基结合形成异源二聚体，介导细胞与细胞外基质（ECM）的相互作用。它与 alpha-1、alpha-2、alpha-10 和 alpha-11 结合形成胶原受体，识别胶原中的 G-F-P-G-E-R 序列；与 alpha-2、alpha-3、alpha-4、alpha-5、alpha-8、alpha-10、alpha-11 和 alpha-V 结合形成纤连蛋白受体，其中 alpha-4/beta-1 识别纤连蛋白可变剪接区 CS-1 和 CS-5 结构域。此外，alpha-5/beta-1 是纤维蛋白原受体，而 alpha-1/beta-1、alpha-2/beta-1、alpha-6/beta-1 和 alpha-7/beta-1 是层粘连蛋白受体（by similar：alpha-6/beta-1 存在于卵母细胞中参与精卵融合）。alpha-4/beta-1 和 alpha-9/beta-1 是 VCAM1 受体，后者还能识别 cytotactin 中的 A-E-I-D-G-I-E-L 序列。alpha-3/beta-1 可结合 epiligrin、thrombospondin 和 CSPG4，并与 LGALS3 协同介导 CSPG4 诱导的内皮细胞迁移。alpha-V/beta-1 是玻连蛋白受体，而 beta-1 整合素普遍识别配体中的 R-G-D 序列。该蛋白参与调控细胞粘附、层粘连蛋白基质沉积、内皮细胞运动、血管生成、成骨细胞矿化结节形成等过程，还能通过结合 KRT1 激活 PKC 等激酶，并与 KRT1、RACK1 形成 SRC 调控平台。在病原体感染中，不同 alpha/beta-1 复合物可作为人类埃可病毒 1/8、巨细胞病毒、EB 病毒、细小病毒 B19、轮状病毒、哺乳动物呼肠孤病毒等的受体，在 HIV-1 感染中通过与 Tat 蛋白结合促进卡波西肉瘤血管生成，还能介导毛霉菌 CotH 蛋白的上皮细胞侵袭。

Free

P05556

Predicted band size: 88 kDa；Observed band size: 120-140 kDa

Protein A affinity purified.

Cell membrane, Cell projection, Cleavage furrow

Non-conjugated

Unmodified

AB_3102502

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000; IHC-P:1:50; FC: 1:50

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) , A431(lane 3(20μg) and HEK293(lane 4(20ug)using Integrin beta 1 (HY-P80418 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Integrin beta-1.AA range:652-701.