2. 抗体
  3. 一抗
  4. 多克隆抗体
  5. MPO 抗体

MPO Antibody 是一个兔来源、无偶联标记、抗 MPO 的 IgG 多克隆抗体。

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规格 价格 是否有货 数量
20 μL ￥ 508.95

￥783.00

 In-stock
50 μL ￥ 731.25

￥1125.00

 In-stock
100 μL ￥ 1170.00

￥1800.00

 In-stock
250 μL   询价  
开学季截至 6.30 日，灰色划线价为牌价

* Please select Quantity before adding items.

实现从蛋白分离到检测的高效衔接！

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

MPO Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MPO.
宿主

Rabbit
克隆性

Polyclonal
分子量
Predicted band size: 84 kDa;
Observed band size: 80-90 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse 预测反应种属: Rat, Dog, Horse, Rabbit, GuineaPig
请注意：预测反应种属仅供参考，不作为质保凭证。
蛋白数据库
基因 ID
免疫原

KLH conjugated synthetic peptide derived from human Myeloperoxidase heavy chain: 678-745/745
应用 & 推荐
稀释比例
应用 稀释比
ELISA
ELISA: 酶联免疫吸附试验
1:5000-10000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-500
IHC-F
IHC-F: 冰冻切片样本的免疫组织化学
1:100-500
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-500
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-500
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL IHC mIHC

  • Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma‌ tissue using MPO antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81219, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using MPO antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81219, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using MPO antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81219, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using MPO antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81219, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using MPO antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81219, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using MPO antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81219, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using MPO antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81219, 1：1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using MPO antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81219, 1：1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cholangiocarcinoma tissue using MPO antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81219, 1：1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using MPO antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81219, 1：1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using MPO antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81219, 1：1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using MPO antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81219, 1：1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景
功能：髓过氧化物酶 (MPO) 是多形核白细胞宿主防御系统的一部分，负责对多种微生物发挥杀菌活性。在受刺激的 PMN 中，MPO 催化次卤酸 (在生理条件下主要为次氯酸) 和其他毒性中间体的生成，这些物质能显著增强 PMN 的杀菌活性 (PubMed:9922160)。MPO 还介导 α-1-微球蛋白的蛋白水解，生成反式 α-1-微球蛋白 (t-α-1-微球蛋白)，后者能有效抑制低密度脂蛋白颗粒的氧化，从而减少血管损伤 (PubMed:25698971)。
亚细胞定位：溶酶体
异构体 & 翻译后修饰：P05164 有 3 个异构体：P05164-1：83869 Da (预测)；P05164-2：73854 Da (预测)；P05164-3：87248 Da (预测)。
亚基：同源二聚体；二硫键连接。每个单体由一条轻链和一条重链组成。与 CP 和 LTF 形成复合物；直接与 CP 相互作用，通过 MPO 保护 CP 的抗氧化特性 (PubMed:23843990)。
RRID
反应种属数据库

Entrez Gene: 4353 Human ; 17523 Mouse ;

SwissProt: P05164 Human ; P11247 Mouse ;

OMIM: 254600 Human

中文名
髓过氧化物酶抗体
同用名
Myeloperoxidase; MPO; 89 kDa myeloperoxidase; 84 kDa yeloperoxidase; Myeloperoxidase heavy chain; EC 1.11.1.7; PERM_HUMAN.
文件资料

COA (192 KB)

抗体使用指南 (1083 KB)

MPO Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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