2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体
  5. STAT5a+b 抗体 (YA054)

STAT5a+b 抗体 (YA054)

目录号: HY-P80904
COA 抗体使用指南 技术支持

STAT5a+b Antibody (YA054) 是一个兔来源、无偶联标记、抗 STAT5a+b 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥470 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

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描述

STAT5a+b Antibody (YA054) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5a+b.
研究背景

STAT5a+b：启用 DNA 结合转录激活剂活性，RNA 聚合酶 II 特异性；RNA 聚合酶 II 顺式调节区序列特异性 DNA 结合活性；和磷酸根离子结合活性。通过 JAK-STAT 参与生长激素受体信号通路和生长调节。作用于多个过程的上游或内部，包括动物器官发育；白细胞增殖的正调节；和调节造血功能。位于细胞质和细胞核中。以多种结构表达，包括消化系统；中枢神经系统;泌尿生殖系统；淋巴系统；以及肝脏和胆道系统。与人类 STAT5A（信号转导和转录激活因子 5A）同源。[由基因组资源联盟提供，2022 年 4 月]
标签

Free
基因 ID
蛋白数据库
中文名
STAT5a+b 抗体 (YA054)
同用名
MGF; STAT5
分子量

Predicted band size: 91 kDa; Observed band size: 91 kDa
纯度

affinity purified
偶联

Non-conjugated
修饰

Unmodified
RRID
研究领域

Signal Transduction
产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
克隆性

Recombinant, Monoclonal
宿主

Rabbit
反应物种

Human, Mouse, Rat
推荐稀释比例

WB: 1:500-1:1,000; IF: 1:50-1:200; IP: 1:20

  • Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
应用

WB, ICC/IF, IP
性状

液体
组分

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
同型

IgG
敏感性

Endogenous
免疫原

Synthetic peptide corresponding to Mouse STAT5A aa600-786.
数据库

Entrez Gene: 6776 Human ; 6777 Human ; 20850 Mouse ; 20851 Mouse ; 24918 Rat 25126

SwissProt: P42229 Human ; P51692 Human ; P42230 Mouse ; P42232 Mouse ; P52632 Rat Q62771

OMIM: 601511 Human

文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

STAT5a+b Antibody (YA054) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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STAT5a+b Antibody (YA054)
目录号：
HY-P80904
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