2. Metabolic Enzyme/Protease Immunology/Inflammation Anti-infection
  3. Endogenous Metabolite NOD-like Receptor (NLR) Interleukin Related Bacterial
  4. ATP disodium salt, 100mM Solution, PCR Grade

ATP disodium salt, 100mM Solution, PCR Grade  (Synonyms: Adenosine 5'-triphosphate disodium salt, 100mM Solution, PCR Grade)

目录号: HY-B2176I
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ATP (Adenosine 5'-triphosphate) disodium salt, 100mM Solution, PCR Grade 是体内能量储存和代谢的重要物质，为代谢提供能量，同时在细胞中作为辅酶发挥作用。ATP disodium salt, 100mM Solution, PCR Grade 是免疫和炎症中重要的内源性信号分子。ATP disodium salt, 100mM Solution, PCR Grade 可激活 NLRP3 炎症小体，并诱导 IL-1β 和趋化因子的分泌。ATP disodium salt, 100mM Solution, PCR Grade 具有抗细菌感染作用，能保护小鼠免受细菌感染。

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ATP disodium salt, 100mM Solution, PCR Grade

ATP disodium salt, 100mM Solution, PCR Grade Chemical Structure

CAS No. : 987-65-5

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1 mL ￥525
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ATP disodium salt, 100mM Solution, PCR Grade 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 126 篇科研文献

WB
Cell Viability Assay
RT-PCR

    ATP disodium salt, 100mM Solution, PCR Grade purchased from MCE. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1 (one experiment, n = 3). Supernatant (Sup) IL-1β (P17) and cellular lysates (Lys) pro-IL-1β; (I) supernatant caspase-1 (P20) and lysates pro-caspase-1.

    ATP disodium salt, 100mM Solution, PCR Grade purchased from MCE. Usage Cited in: Transl Res. 2023 May:255:1-13.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP disodium salt, 100mM Solution, PCR Grade purchased from MCE. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP disodium salt, 100mM Solution, PCR Grade purchased from MCE. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP disodium salt, 100mM Solution, PCR Grade purchased from MCE. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs and BMMs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP or 100 ng/mL for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP disodium salt, 100mM Solution, PCR Grade purchased from MCE. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

    查看 NOD-like Receptor (NLR) 亚型特异性产品:

    查看所有亚型
    NLRP3 NLRP1 NOD1 NOD2

    查看 Interleukin Related 亚型特异性产品:

    查看所有亚型
    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    ATP (Adenosine 5'-triphosphate) disodium salt, 100mM Solution, PCR Grade is a central component of energy storage and metabolism in vivo. ATP disodium salt, 100mM Solution, PCR Grade provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt, 100mM Solution, PCR Grade is an important endogenous signaling molecule in immunity and inflammation. ATP disodium salt, 100mM Solution, PCR Grade can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP disodium salt, 100mM Solution, PCR Grade has anti-bacterial infection effects and can protect mice against bacterial infection in mice[1][2][3][4].

    IC50 & Target[4]

    NLRP3 inflammasome

     

    IL-1β

     

    体外研究
    (In Vitro)

    ATP (5 mM；1 小时) disodium salt, 100mM Solution, PCR Grade 与 LPS (1 μg/mL) 共同处理对 HGFs 中 NLRP3 炎性体的激活具有协同作用[3]。
    ATP (2 mM；0.5-24 小时) disodium salt, 100mM Solution, PCR Grade 以 caspase-1 激活依赖性方式诱导 BMDM 分泌 IL-1β、KC 和 MIP-2[4]。
    ATP (2mM) disodium salt, 100mM Solution, PCR Grade 促进中性粒细胞趋化作用[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP disodium salt, 100mM Solution, PCR Grade 相关抗体:
    体内研究
    (In Vivo)

    ATP (50 mg/kg，腹腔注射，细菌攻击前 1-24 小时) disodium salt, 100mM Solution, PCR Grade 可保护小鼠免受细菌感染，如大肠杆菌和金黄色葡萄球菌[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice infected with E. coli and S. aureus (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, 1, 4, 24 h before bacterial (E. coli) challenge
    Result: Reduced peritoneal bacterial counts.
    Increased the levels of IL-1β, KC and MIP-2 in the peritoneal lavage fluid.
    Recruited neutrophils to the peritoneum.
    分子量

    551.14

    Formula

    C10H14N5Na2O13P3
    CAS 号
    性状

    液体

    颜色

    Colorless to light yellow

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Solution, -20°C, 2 years
    纯度 & 产品资料

    COA (267 KB)

    产品使用指南 (1538 KB)
    参考文献

    ATP disodium salt, 100mM Solution, PCR Grade 相关分类

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP disodium salt, 100mM Solution, PCR Grade987-65-5Adenosine 5'-triphosphate disodium salt, 100mM Solution, PCR GradeEndogenous MetaboliteNOD-like Receptor (NLR)Interleukin RelatedBacterialILInhibitorinhibitorinhibit

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