2. Others
  3. Biochemical Assay Reagents
  4. BC-GLA-NHS

BC-GLA-NHS (CLIP-GLA-NHS) 是一种 NHS 标记的 CLIP tag，属于胺反应性交联剂，可实现位点特异性蛋白标记。BC-GLA-NHS 的 NHS 酯与目标分子 (如抗体、量子点) 的伯胺在 pH 7.5-8.5 缓冲液中反应，形成稳定的酰胺键。偶联后的 BC-分子与 CLIP-tag 融合蛋白的活性位点 (Cys) 发生亲核取代反应，释放苄基胞嘧啶，形成不可逆的硫醚键，实现共价标记。BC-GLA-NHS 可用于点击化学标记。

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BC-GLA-NHS

BC-GLA-NHS Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

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BC-GLA-NHS 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

BC-GLA-NHS (CLIP-GLA-NHS) is a NHS-labeled CLIP tag, which is an amine-reactive crosslinker and can achieve site-specific protein labeling. The NHS ester of BC-GLA-NHS reacts with the primary amine of the target molecule (such as antibodies, quantum dots) in a pH 7.5-8.5 buffer solution to form a stable amide bond. After coupling, the BC molecule undergoes a nucleophilic substitution reaction with the active site (Cys) of the CLIP-tagged fusion protein, releasing benzylcytosine and forming an irreversible thioether bond, achieving covalent labeling. BC-GLA-NHS can be used for click chemistry labeling[1].

分子量

441.44

Formula

C21H23N5O6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

BC-GLA-NHS 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BC-GLA-NHSCLIP-GLA-NHSBiochemical Assay ReagentsFluorescent probesCovalent labelingProtein modificationsCell imagingSNAP-tagCLIP-tagInhibitorinhibitorinhibit

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目录号：
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