CFDA-SE (solution) 

CFDA-SE (solution) is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus^[1]. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells^[1].
Solvent and concentration: DMSO: 5 mM

CFDA-SE 工作液的配制
可根据实际情况稀释相应的储备液。注意，如果溶剂为 DMSO，则必须考虑 DMSO 的细胞毒性，并应制备溶剂对照；如果溶剂为纯水，则工作液在加入细胞前需要过滤并除菌。
细胞染色
2.1 悬浮细胞：4°C，1000 g 离心 3-5 分钟，弃上清。PBS 洗涤 2 次，每次 5 分钟。
贴壁细胞：弃去细胞培养基，加入胰蛋白酶解离细胞，制成单细胞悬液。4°C 1000 g 离心 3-5 分钟，弃上清。PBS 洗涤 2 次，每次 5 分钟。
2.2 加入 1 mL CFDA-SE 工作液，室温孵育 30 分钟。
2.3 4°C，400 g 离心 3-4 分钟
2.4 PBS 洗涤 2 次，每次 5 分钟。
2.5 用无血清细胞培养基或 PBS 重悬细胞，荧光显微镜或流式细胞仪检测。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1114.93

C₅₈H₃₈N₂O₂₂

150347-59-4

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1;26(5):571-577.  [Content Brief]

  [2]. Cui J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021 Jun;25(12):5586-5601.  [Content Brief]

  [3]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.  [Content Brief]

  [4]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.  [Content Brief]