2. Anti-infection
  3. Bacterial
  4. CyLip-20

CyLip-20 是一种环脂类抗菌肽，靶向革兰氏阳性菌和革兰氏阴性菌。CyLip-20 的溶血活性低且体内毒性轻微。CyLip-20 通过结合细菌脂多糖 (LPS) 破坏细菌外膜、内膜及细胞质膜完整性，引发膜通透化、去极化及细胞内容物泄漏，并抑制细菌生物膜形成。CyLip-20 在动物模型中能降低 MRSA 感染小鼠皮肤伤口的细菌载量、促进伤口愈合、降低炎症因子水平且减轻炎性细胞浸润。CyLip-20 可用于 MRSA 皮肤伤口感染的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Custom Peptide Synthesis

CyLip-20

CyLip-20 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

CyLip-20 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

CyLip-20 is a cyclic lipopeptide antimicrobial peptide that targets Gram-positive and Gram-negative bacteria. CyLip-20 exhibits low hemolytic activity and mild in vivo toxicity. CyLip-20 disrupts the integrity of bacterial outer membrane, inner membrane and cytoplasmic membrane by binding to bacterial lipopolysaccharide (LPS), triggering membrane permeabilization, depolarization and leakage of intracellular contents, and inhibits bacterial biofilm formation. In animal models, CyLip-20 reduces the bacterial load in skin wounds of mice infected with MRSA, promotes wound healing, decreases the levels of inflammatory cytokines and reduces inflammatory cell infiltration. CyLip-20 can be used in research related to MRSA skin wound infections[1].
体外研究
(In Vitro)

CyLip-20 (4-32 μM；18-24 h) 对革兰氏阳性菌和革兰氏阴性菌 (包括金黄色葡萄球菌 ATCC 25923、大肠杆菌 ATCC 25922 及铜绿假单胞菌 ATCC 27853) 表现出广谱抗菌活性，其 GMall 为 5.19 μM[1]。
CyLip-20 (10 mM；0-48 h) 在小鼠血清中保持高稳定性，半衰期超过 48 h[1]。
CyLip-20 (1 mM；与蛋白酶共孵育 6 小时) 对胰蛋白酶和胰凝乳蛋白酶 (0.02-200 μg/mL) 具有高度稳定性，均能维持抗菌活性[1]。
CyLip-20 (1/2×MIC-4×MIC；24 h) 对金黄色葡萄球菌 (S. aureus)、大肠杆菌 (E. coli)和铜绿假单胞菌 (P. aeruginosa)的生物膜形成具有浓度依赖性的抑制作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CyLip-20 相关抗体:

Cell Viability AssayWestern Blot AnalysisCell Proliferation AssayApoptosis AnalysisCell Cytotoxicity AssayCell Cycle AnalysisRT-PCRCell Autophagy AssayImmunofluorescenceCell Differentiation AssayCell Invasion AssayCell Migration Assay Real Time qPCRELISA Assay[1]

Cell Line: S. aureus ATCC 25923, B. subtilis ATCC 23857, S. epidermidis ATCC 12228, E. faecalis ATCC 29212, E. coli ATCC 25922, K. pneumoniae ATCC 700603, P. aeruginosa ATCC 27853, A. baumannii ATCC 19606
Concentration: MIC
Incubation Time: 24 h
Result: Showed MIC values of 4 μM for S. aureus ATCC 25923, B. subtilis ATCC 23857, S. epidermidis ATCC 12228, E. coli ATCC 25922, K. pneumoniae ATCC 700603, P. aeruginosa ATCC 27853, A. baumannii ATCC 19606, and showed a MIC value of 32 μM for E. faecalis ATCC 29212.
Inhibited the biofilm formation in a concentration-dependent manner against S. aureus, E. coli, and P. aeruginosa.
体内研究
(In Vivo)

CyLip-20 (2 mg/mL, 10 μL；经局部给药；每间隔 1 小时给药 1 次，共 6 次) 可有效降低 BALB/C 小鼠耐甲氧西林金黄色葡萄球菌 (MRSA) 感染皮肤伤口中的MRSA载菌量，促进伤口愈合并改善组织修复[1]。
CyLip-20 (30-130 mg/kg；腹腔注射；单剂量) 在雄性 BALB/C 小鼠中的 LD50 为 62.52 mg/kg，体内毒性低于 Polymyxin B (LD50=18.90 mg/kg)[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MRSA skin wound infection model in BALB/C mouse (male, 6-8 weeks old, 20 ± 2 g)[1]
Dosage: 2 mg/mL; 10 μL
Administration: topical; 6 times at 1 h intervals; observed the wound healing at days 12.
Result: Reduced MRSA bacterial load at the wound site.
Significantly promoted wound healing, with wound area smaller than model and vancomycin groups by day 4, scabs almost fell off by day 6, wounds almost completely healed by day 8.
Reduced serum TNF-α levels.
Rendered skin tissue structure closest to healthy control with no obvious cell swelling, necrosis, or abnormal proliferation.
Reduced macrophage and neutrophil infiltration at the wound site
分子量

1544.88

Formula

C78H117N19O14
Sequence

C8-{Dab}-{Dab}-Trp-{d-Tyr}-cyclo(Lys-{Dab}-{Dab}-Trp-{d-Leu}-{d-Leu}-Glu)
Sequence Shortening

C8-{Dab}-{Dab}-W-{d-Tyr}-cyclo(K-{Dab}-{Dab}-W-{d-Leu}-{d-Leu}-E)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

CyLip-20 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CyLip-20CyLip20CyLip 20BacterialE. coliMRSAbacterial lipopolysaccharide (LPS)bacterial inner membraneGram-negative bacteriaS. aureuscytoplasmic membranebacterial outer membraneP. aeruginosaGram-positive bacteriaInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
CyLip-20
目录号：
HY-P11582
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z