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  3. DFHBI-BI

DFHBI-BI 是绿色荧光蛋白发色团的模拟物。DFHBI-BI 可以与 Broccoli (一种荧光 RNA 适配体)结合并被其激活,且结合亲和力更高。在哺乳动物细胞中,DFHBI-BI 可以防止 Broccoli 在 37°C 下发生热变性,从而形成更多具有荧光特性的 Broccoli-BI 复合物。其荧光波长为 559/635 nm。

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DFHBI-BI

DFHBI-BI Chemical Structure

CAS No. : 2561482-42-4

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规格 价格 是否有货 数量
1 mg ¥2430
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Other Forms of DFHBI-BI:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DFHBI-BI is a mimic of the green fluorescence protein chromophore. DFHBI-BI can be bound and activated by Broccoli ( a fluorogenic RNA aptamer) with higher affinity. DFHBI-BI prevents thermal unfolding of Broccoli at 37°C in mammalian cells, subsequently forming more fluorescent Broccoli-BI complexes. The fluorescence wavelength is 559/635 nm[1].

体外研究
(In Vitro)

DFHBI-BI (10 μM) 染料对细胞染色 2 小时后,细胞的荧光强度较低。​​ DFHBI-BI 应用于表达 Broccoli 环状 RNA 的 HEK293T 细胞后,观察到荧光强度有所增加[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

368.34

Formula

C19H14F2N4O2

CAS 号
性状

固体

颜色

brownish yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
纯度 & 产品资料
参考文献
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  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
DFHBI-BI
目录号:
HY-D2282
需求量: