2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. Ethidium bromide (solution)

Ethidium bromide (solution) 常用作分子生物实验学中的荧光标记 (核酸染色) ，如琼脂糖凝胶电泳。
溶剂及浓度：无菌水：10 mg/mL

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Ethidium bromide (solution)

Ethidium bromide (solution) Chemical Structure

CAS No. : 1239-45-8

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Other Forms of Ethidium bromide (solution):

Ethidium bromide (solution) 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ethidium bromide (solution) is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Solvent and Concentration: Sterile water: 10 mg/Ml

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A2780 IC50
0.1 μM
Compound: Ethidium
Concentration required to inhibit 50% of human carcinoma A2780 cell lines
Concentration required to inhibit 50% of human carcinoma A2780 cell lines
[PMID: 15501053]
CH1 IC50
0.4 μM
Compound: Ethidium
Concentration required to inhibit 50% of human carcinoma CH1 cell lines
Concentration required to inhibit 50% of human carcinoma CH1 cell lines
[PMID: 15501053]
MDA-MB-231 IC50
2.33 μM
Compound: Ethidium bromide
Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA
Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA
[PMID: 25965778]
MGC-803 IC50
2.1 μM
Compound: Ethidium bromide
Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA
Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA
[PMID: 24119869]
MGC-803 IC50
2.1 μM
Compound: Ethidium bromide
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA
[PMID: 25554922]
MGC-803 IC50
2.18 μM
Compound: 2
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method
[PMID: 26883149]
MGC-803 IC50
2.39 μM
Compound: Ethidium bromide
Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
[PMID: 23265905]
MGC-803 IC50
2.47 μM
Compound: Ethidium bromide
Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
[PMID: 22405648]
NIH3T3 IC50
134.87 μM
Compound: Ethidium bromide
Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay
[PMID: 24286761]
SGC-7901 IC50
2.5 μM
Compound: Ethidium bromide
Inhibition of telomerase in human SGC7901 cells by TRAP assay
Inhibition of telomerase in human SGC7901 cells by TRAP assay
[PMID: 20800480]
SGC-7901 IC50
2.5 μM
Compound: Ethidium bromide
Inhibition of telomerase in human SGC7901 cells by TRAP assay
Inhibition of telomerase in human SGC7901 cells by TRAP assay
[PMID: 20947362]
SGC-7901 IC50
2.6 μM
Compound: Ethidium bromide
Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP
Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP
[PMID: 21486698]
SGC-7901 IC50
2.6 μM
Compound: Ethidium bromide
Inhibition of telomerase in human SGC7901 cells by TRAP assay
Inhibition of telomerase in human SGC7901 cells by TRAP assay
[PMID: 23312949]
SK-OV-3 IC50
4.5 μM
Compound: Ethidium
Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines
Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines
[PMID: 15501053]
SW 1116 IC50
2.71 μM
Compound: Ethidium bromide
Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay
Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay
[PMID: 24286761]
体外研究
(In Vitro)

指南 (以下是我们推荐的协议。该协议仅提供指南，应根据您的具体需求进行修改)。
琼脂糖凝胶的制备：
1. 琼脂糖凝胶的常用浓度为 0.5％ 至 2.5％，具体取决于需要分离的带的大小。
2. 将琼脂糖粉与 1 X TAE/TBE 混合。
3. 用微波炉加热 1-3 分钟，直至琼脂糖完全溶解 (注意：不要煮得太沸腾)。
4. 使溶液冷却后再凝固。
5. 加入 Ethidium bromide (EtBr) 至最终浓度约为 0.2-0.5 μg/mL (原液通常为 10 mg/mL，需要 5 μL 原液/100 mL 凝胶)。
6. Ethidium bromide 与 DNA 结合，在紫外线 (UV) 光下即可显现 DNA。
注意：Ethidium bromide 是已知的诱变剂。请注意加强防护。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Ethidium bromide (solution) 相关抗体:
分子量

394.31

Formula

C21H20BrN3
CAS 号
性状

液体（密度：1.3739 g/cm3）

颜色

Colorless to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Ethidium bromide (solution) 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethidium bromide (solution)1239-45-8DNA StainInhibitorinhibitorinhibit

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