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  4. Firefly luciferase mRNA-LNP

Firefly luciferase mRNA-LNP  (Synonyms: 萤火虫荧光素酶mRNA-LNP)

目录号: HY-153229
COA 产品使用指南 技术支持

Firefly luciferase mRNA-LNP 是一种含有萤火虫荧光素酶 mRNA 的脂质纳米颗粒 (LNP)。Firefly luciferase mRNA-LNP 具有稳定的纳米结构，其中 LNP 在有效保护和运输 mRNA 至细胞内方面发挥着关键作用。Firefly luciferase mRNA-LNP 中的荧光素酶是一种用于基因调控和功能研究的生物发光报告基因。Firefly luciferase mRNA-LNP 进入细胞后表达萤火虫荧光素酶蛋白，常用于启动子活性检测或双荧光分子互补实验。Firefly luciferase mRNA-LNP 被用于 RNA 递送、细胞活力和翻译效率等方面的研究。

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Firefly luciferase mRNA-LNP

Firefly luciferase mRNA-LNP Chemical Structure

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规格 价格 是否有货 数量
100 μg (135.199 μg/mL * 0.74mL in buffer) ￥4000
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500 μg (135.199 μg/mL * 3.7mL in buffer) 现货 询价

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Customer Review

Firefly luciferase mRNA-LNP 相关抗体

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Firefly luciferase mRNA-LNP is a lipid nanoparticle (LNP) containing firefly luciferase mRNA. Firefly luciferase mRNA-LNP exhibits a stable nanostructure, in which LNP plays a key role in effectively protecting and transporting mRNA to cells. Luciferase is a bioluminescent reporter gene for gene regulation and functional studies. Firefly Luciferase mRNA-LNP expresses firefly luciferase protein after entering the cells, which is often used for promoter activity detection or dual fluorescent molecular complementation experiments. Firefly luciferase mRNA-LNP is studied in research for RNA delivery, cell viability, and translation efficiency[1][2].

体外研究
(In Vitro)

Firefly luciferase mRNA-LNP (5-20 ng，24 小时) 在 HepG2 细胞中显著提高转染效率，且呈剂量依赖性[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Firefly luciferase mRNA-LNP 相关抗体:
体内研究
(In Vivo)

Firefly luciferase mRNA-LNP (2 μg，静脉注射，单剂量) 可使 C57BL/6 小鼠达到总发光通量[3]。
Firefly luciferase mRNA-LNP (1 μg，肌肉注射，第 1 天和第 21 天) 与 ALC-0315 或 SM-102 配制而成，在 BALB/c 小鼠中，SM-102 的荧光素酶蛋白表达水平比 ALC-0315 和 cKK-E12 高 60%[4]。
Firefly luciferase mRNA-LNP (5-20 μg，肌肉注射，单剂量) 可诱导成年 Balb/c 雄性小鼠下丘脑中 Aif1 的激活，但不诱导前额叶皮质中 Aif1 的激活[5]。
Firefly luciferase mRNA-LNP (5 μg，肌肉注射/静脉注射/腹腔注射/皮下注射，单剂量) 可在 BALB/c 小鼠模型中引起大量的荧光素酶活性以及肝脏中的高水平蛋白质生成，并在肺和肌肉中产生显著的生物发光信号[6]。
Firefly luciferase mRNA-LNP (5 μg，i.d./s.c.，单剂量) 仅在 BALB/c 小鼠模型中注射部位产生蛋白质[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c mice aged 6 weeks[1]
Dosage: 0.1, 1.0, 5.0 μg
Administration: Intradermal injection (i.d.)/Intraperitoneal injection (i.p.)/Subcutaneous injection (s.c.)/Intramuscular injection (i.m.)/Intravenous injection (i.v.)/Intratracheal injection (i.t.)
Result: Could not measure signal when mice were injected with 0.1 μg of mRNA-LNPs.
Resulted in the highest amount of peak protein production and the shortest duration of expression with intravenous delivery.
Did not result in measurable luciferase expression in the liver or other anatomical sites with subcutaneous delivery.
Resulted in the longest duration of translation with intramuscular and intradermal delivery.
Allowed saturation of the uptake or translational capacity of the intradermal space with intradermal delivery.
Observed linear dose response with intravenous injection.
Led to similar levels of peak translation at 4 h post injection with all three doses, but the increased doses extended the duration of translation from 5 (1.0 μg) to 10 (5.0 μg) days.
性状

液体

颜色

Colorless to light yellow

中文名称

萤火虫荧光素酶mRNA-LNP
运输条件

Shipping with dry ice.
储存方式

-80°C
纯度 & 产品资料

COA (254 KB)

产品使用指南 (1538 KB)
参考文献

Firefly luciferase mRNA-LNP 相关分类

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Firefly luciferase mRNA-LNP萤火虫荧光素酶mRNA-LNPBiochemical Assay ReagentsmRNAlipid nanoparticlebioluminescenttranslation efficiencyInhibitorinhibitorinhibit

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