2. Protein Tyrosine Kinase/RTK Stem Cell/Wnt MAPK/ERK Pathway
  3. FGFR ERK
  4. GP369

GP369 是一种人源化 FGFR2-IIIb 特异性抗体。GP369 显著抑制肿瘤细胞的增殖。GP369 可以显著抑制 FGFR2 的磷酸化和下游信号通路。GP369 可用于癌症如胃癌和乳腺癌的研究。

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GP369

GP369 Chemical Structure

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Customer Review

GP369 相关抗体

Top Publications Citing Use of Products

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ERK ERK1 ERK2 ERK5 ERK8

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GP369 is a humanized FGFR2-IIIb-specific antibody. GP369 significantly inhibits the proliferation of tumor cells. GP369 can significantly inhibit phosphorylation of FGFR2 and downstream signaling pathways. GP369 can be used for research on cancer such as gastric cancer and breast cancer[1].

反应种属

Human
IC50 & Target[1]

FGFR2

 

体外研究
(In Vitro)

GP369 (0.001-1000 nM，2-5 天) 可以显著抑制多种细胞的增殖，包括 FDCP-1，SUM52PE 和 MCF7 细胞[1]。
GP369 (0.001-100 nM，2 天) 抑制 FDCP-1 (IC50 = 1.4 nM) 细胞中的 FGFR2-IIIb 驱动的增殖，对 FGFR2-IIIIc 没有影响[1]。
GP369 (30 μg/mL，3 天) 抑制 FGF7 (25 ng/mL) 在 MCF7 细胞中诱导的增殖[1]。
GP369 (10 μg/mL，1-48 小时) 抑制 Ba/F3 和 SNU-16 细胞中 FGFR2 和 ERK1/2 磷酸化和下游信号传导[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GP369 相关抗体:

Cell Proliferation Assay[1]

Cell Line: FDCP-1 cells
Concentration: 0.001-100 nM
Incubation Time: 2 days
Result: Significantly inhibited FGFR2 IIIb driven proliferation, including FGFR2 IIIb WT, FGFR2 IIIb N550K and FGFR2 IIIb S252W.

Western Blot Analysis[1]

Cell Line: SNU-16 cells
Concentration: 10 μg/mL
Incubation Time: 2, 6, 24 and 48 h
Result: Reduced the level of FGFR2 protein.

Western Blot Analysis[1]

Cell Line: Ba/F3 cells and SNU-16 cells
Concentration: 5 μg/mL
Incubation Time: Incubated for 1 h and then combinated with FGF7 for 15 min
Result: Significantly inhibited FGF7 induced phosphorylation and downstream protein levels, including p-FGFR and p-ERK1/2.
体内研究
(In Vivo)

GP369 (2-20 mg/kg；腹腔注射；每周两次；持续 43 天) 显著抑制了携带 SNU-16 异种移植物的 C.B-17 严重联合免疫缺陷 (SCID) 小鼠的肿瘤生长，降低了 FGFR2 磷酸化和蛋白质水平[1]。
GP369 (20 mg/kg；腹腔注射；每周两次；持续 27 天) 在携带 MFM-223 异种移植物的 NCr 裸鼠中实现了肿瘤阻滞，并显著降低了 FGFR2 磷酸化和蛋白质水平[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C.B-17 SCID mice bearing 5×106 SNU-16 cells (10 weeks)[1]
Dosage: 2, 5, 10 and 20 mg/kg
Administration: Intraperitoneal injection (i.p.); twice weekly for 43 days
Result: Significantly inhibited tumor volume.
Significantly reduced the levels of FGFR2 and p-FGFR.
Animal Model: 1×107 MFM-223 cells injected female NCr nude mice (5 weeks)[1]
Dosage: 20 mg/kg
Administration: Intraperitoneal injection (i.p.); twice weekly for 27 days
Result: Significantly inhibited tumor volume.
Significantly reduced the levels of FGFR2 and p-FGFR.
基因 ID

2263  [NCBI]

Accession
靶点

FGFR2/CD332
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GP369 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GP369GP 369GP-369FGFRERKFibroblast growth factor receptorExtracellular signal regulated kinasesFGFR2ERK1/2gastric cancerbreast cancerInhibitorinhibitorinhibit

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