HMRef-αMan 

HMRef-αMan is a substrate-based green fluorescent probe (Ex/Em=465 nm/515 nm) targeting MAN2C1 (α-mannosidase). HMRef-αMan can be specifically cleaved by MAN2C1 to generate a highly fluorescent product, which thus gets activated to produce green fluorescence in malignant breast tissues, benign lesions and living cancer cells. The signal intensity of HMRef-αMan is directly correlated with MAN2C1 activity, and it can effectively detect tiny breast cancer lesions with a diameter of less than 1 mm. When used in combination with the red-emitting γ-glutamyl transpeptidase (GGT) probe gGlu-2OMe SiR600 (HY-D3188), HMRef-αMan enables precise optical differentiation of breast tissue types via a dual-color imaging strategy. HMRef-αMan has been widely used in the research of breast diseases such as breast cancer, fibroadenoma, phyllodes tumor and various types of papilloma^[1].

HMRef-αMan (10 μM; 1 h) 可在多种活的培养人癌细胞系中对 α-mannosidase 活性进行可视化^[1]。
HMRef-αMan (50 μM; 1-30 min) 可选择性且时间依赖性地在手术切除的人浸润性导管癌 (IDC) 和导管原位癌 (DCIS) 组织中激活荧光，实现乳腺癌检测，灵敏度达 90%、特异性达 100%^[1]。
HMRef-αMan (50 μM; 5-30 min) 可在 15 分钟内快速显影手术切除标本中经病理证实的微小人类浸润性导管癌 (IDC) 和导管原位癌 (DCIS) 病灶，且荧光激活仅局限于癌组织区域^[1]。
HMRef-αMan (50 μM; 5-30 min) 可在手术切除的人类良性乳腺病变 (FA、PT、ICP、IDP) 中激活荧光，实现与正常组织的区分，对 FA 的灵敏度和特异性均达 100%^[1]。
HMRef-αMan (50 μM; 15 min; Ex/Em=465 nm/515 nm)，与红色荧光探针 gGlu-2OMe SiR600 (HY-D3188) (Ex/Em=570 nm/610 nm) 联合使用时，可对手术切除的人正常乳腺组织、IDC/DCIS 及 FA 进行光学区分^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

561.50

C₂₈H₂₆F₃NO₈

2484831-02-7

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Fujita K, et al. Rapid and accurate visualization of breast tumors with a fluorescent probe targeting α-mannosidase 2C1[J]. ACS central science, 2020, 6(12): 2217-2227.