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Laurdan (solution) 是一种膜透性荧光探针,它对与之结合的细胞膜磷脂相具有光谱敏感性。Laurdan 的广义极化 (GP) 定量可用于磷脂相的鉴定。
溶剂及浓度:DMSO:5 mM

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Laurdan (solution)

Laurdan (solution) Chemical Structure

CAS No. : 74515-25-6

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规格 价格 是否有货 数量
1 mL ¥180
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10 mL   询价  

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Laurdan (solution) is a membrane-permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound. Quantitation of generalized polarization (GP) of Laurdan can be used to identify phospholipid phase.
Solvent and concentration: DMSO: 5 mM

体外研究
(In Vitro)

Laurdan 染色液配置
[1]
1.1 工作液的配制
可根据实际情况稀释相应的储备液。注意,如果溶剂为 DMSO,则必须考虑 DMSO 的细胞毒性,并应制备溶剂对照;如果溶剂为纯水,则工作液在加入细胞前需要过滤并除菌。
Laurdan 染色
1) 将细胞培养细胞完全培养基中,培养基中补充 5 mL Pen-Strep 和 2.5 mL 1 M HEPES。
2) 将新鲜分离的细胞接种到涂有纤连蛋白的 35 mm Mattek 玻璃底培养皿中。
3) 将细胞在 37°C 下与 Laurdan 一起孵育 40 min。
4) 利用 LSM710 META 激光扫描显微镜观察。
5) SimFCS 软件处理,通过测量荧光素 (pH 11) 来校准系统和 FLIM 数据的相量图。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

353.55

Formula

C24H35NO

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

Laurdan (solution) 相关分类

  • 摩尔计算器

  • 稀释计算器

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Laurdan (solution)
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HY-DY1025
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