[Studies on chemical constituents in the mycelia from fermented culture of Flammulina velutipes]

Objective: To study the chemical constituents from the mycelia of Flammulina velutipes.

Method: The compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of chemical evidences and spectral analysis (IR, EI-MS, 1H-NMR, 13C-NMR).

Result: Five compounds were identified as 5alpha,8alpha-epidioxy-(22E,24R)-ergost-6,22-dien-3beta-ol (1), ergosta-4,6,8(14),22-tetraen-3-one (2), sterpuric acid (3), mannitol (4), ribitol (5).

Conclusion: The compounds (2)-(5) were isolated for the first time from the mycelia of Flammulina velutipes.