2. Academic Validation
  3. Fontimonas thermophila Alkane Monooxygenase (FtAlkB) Is an Alkyl Fluoride Dehalogenase

Fontimonas thermophila Alkane Monooxygenase (FtAlkB) Is an Alkyl Fluoride Dehalogenase

  • J Am Chem Soc. 2025 Mar 19;147(11):9085-9090. doi: 10.1021/jacs.5c00386.
Lauren Hendricks 1,  Clorice R Reinhardt 2,  Tierani Green 1,  Lily Kunczynski 1,  August Jaunzarins Roberts 1,  Naomi Miller 1,  Noga Rafalin 1,  Heather J Kulik 2 3,  John T Groves 4,  Rachel N Austin 1
Affiliations

Affiliations

  • 1 Department of Chemistry, Barnard College of Columbia University, 3009 Broadway, New York, New York 10027, United States.
  • 2 Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
  • 3 Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
  • 4 Department of Chemistry, Frick Laboratory, Princeton University, Princeton, New Jersey 08544, United States.
PMID: 40052925 DOI: 10.1021/jacs.5c00386
Abstract

Purified alkane monooxygenase (AlkB) from Fontimonas thermophila (FtAlkB) catalyzes the defluorination of 1-fluorooctane, producing octanal, which is partially reduced under the reaction conditions to generate 1-octanol. This reaction occurs preferentially at the monofluorinated methyl group, with only a minor amount of oxidation at the nonfluorinated end of the molecule. The dehalogenation chemistry is specific to 1-fluorooctane, as neither 1-chlorooctane or 1-bromooctane are dehalogenated to an appreciable extent. Furthermore, P. putida cells containing the structurally related AlkB (PpAlkB) along with the full set of genes required for alkane metabolism, utilize 1-fluorooctane as their sole source of carbon with growth rates comparable to those for cells grown with octane.

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