2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. Methional

Methional 是一种醛脱氢酶 (ALDH) 底物，可作为酿酒酵母 ALDH 的底物。Methional 可诱导 IL-3 依赖型小鼠淋巴细胞发生细胞凋亡与 DNA 双链断裂。Methional 可作为自由基清除剂和抗氧化剂。Methional 可用于小鼠淋巴细胞凋亡的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Methional

Methional Chemical Structure

CAS No. : 3268-49-3

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

Methional 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Methional is an aldehyde dehydrogenase (ALDH) substrate that can be found as a substrate for baker's yeast ALDH. Methional induces apoptosis and DNA double-strand breaks in IL-3-dependent murine lymphoid cells. Methional acts as a free-radical scavenger and antioxidant. Methional can be used for the research of murine lymphoid cell apoptosis[1][2][3].

体外研究
(In Vitro)

Methional (0.01-1 mM；处理 3 天) 可直接抑制 HeLa 细胞增殖，其 IC50 为 100 μM[1]。
Methional (200-800 μM；作用 8 小时) 可诱导 BAF3小鼠淋巴样细胞发生 DNA 片段化，其中 600 μM 作用 8 小时可使非沉降性 DNA 增加 2-3 倍，这与 IL3 剥夺的效应相似[1]。
Methional (600 μM；处理 8 小时) 可在 G₁/S 和 G₂/M 期的 BAF₃小鼠淋巴样细胞中诱导广泛的 DNA 双链断裂，该效应在 40% 的细胞中发生[1]。
Methional (600 μM；作用 8 h) 可在 BAF3小鼠淋巴样细胞中诱导典型的凋亡 DNA 梯状条带模式[1]。
Methional (0.018-2.3 mM; 20 min) 是酿酒酵母 ALDH 的底物，其 Km 值为 1.1 mM[1]。
Methional (0.2 mM；2 h) 可通过 Fenton 反应转化为 MDA，0.2 mM 的 Methional 经该反应可生成 3 nmol MDA[1]。
Methional (由 14.5 nmol [3,4-14C]MTOB 生成；孵育 8 小时) 在 BAF3小鼠淋巴样细胞中被代谢为 MDA，且在 IL3 剥夺的细胞中，MDA/methional 比值 (0.94) 高于 IL3充足的细胞 (0.54)[1]。
Methional (600 μM；处理 4-8 h) 可在处理 4 小时后使 49% 的 G2/M 期同步化 BAF3 b0 小鼠淋巴样细胞发生 DNA 链断裂，处理 8 小时后该比例达 94%；且仅处理 4 小时，到第 8 小时时即可诱导 81% 的细胞发生 DNA 链断裂[2]。
Methional (600 μM；作用 4 h) 不会将 G₁期 BAF3 b0 小鼠淋巴样细胞诱导进入凋亡状态，因为处理后 G₁ 期细胞群保持稳定，而 S 期细胞群有所增加[2]。
Methional (0.005%-0.1%；60℃ 储存) 可在 60℃条件下稳定红花油，抑制其自氧化；浓度为 0.1% 时活性显著，即使浓度低至 0.005% 仍具备有效活性[3]。
Methional monomer、dimer 及 trimer (0.01%；60℃ 储存) 在添加量为 0.01% 时，于 60℃ 条件下均在红花油中表现出有效的抗氧化活性[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Methional 相关抗体:

Cell Proliferation Assay[1]

Cell Line: HeLa cells
Concentration: 0.01-1 mM
Incubation Time: 3 days
Result: Inhibited HeLa cell growth with an IC50 of 100 μM; showed lower IC50 than IC50 values (>1.6 mM) for other MTOB-derived metabolites (MTPA, methionol, and Met).

Apoptosis Analysis[1]

Cell Line: BAF₃ murine lymphoid cells
Concentration: 200-800 μM; 600 μM
Incubation Time: 8 h
Result: Induced a time-dependent increase in non-sedimentable DNA up to 8 h and a concentration-dependent increase up to 800 μM; Increased non-sedimentable DNA by 2-3-fold after treatment with 600 μM for 8 h, similar to the increase seen in IL₃-deprived BAF₃ cells.

Apoptosis Analysis[1]

Cell Line: BAF₃ murine lymphoid cells
Concentration: 600 μM
Incubation Time: 8 h
Result: Induced extensive double-strand breaks in 40% of treated cells, distributed across G₁/S and G₂/M phases, with increased green fluorescence from avidin-FITC.\nInduced the typical apoptotic ladder pattern in cellular DNA, which was absent in control, MTOB-treated, or propanal-treated cells.

Cell Cycle Analysis[2]

Cell Line: G1-phase BAF3 b0 murine lymphoid cells
Concentration: 600 μM
Incubation Time: 4 h (added at 3 h post-SK&F 96365 release, examined at 7 h)
Result: Maintained stable G1-phase cell population (37% at 3 h vs. 39% at 7 h; vs. 50% in non-treated cells at 7 h); increased S-phase cell population from 7% in non-treated cells to 19% in methional-treated cells at 7 h.
分子量

104.17

Formula

C4H8OS
CAS 号
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Methional 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methional3268-49-3Reactive Oxygen Species (ROS)methionine salvage pathwaybaker's yeastmalondialdehydeapoptosisDNA double-strand breaksmurine lymphoid cellsHeLa cellmitochondrial metabolitealdehyde dehydrogenaseNADInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Methional
目录号：
HY-W540391
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z