N-Methylmesoporphyrin IX (solution) 

N-Methylmesoporphyrin IX (NMM) (solution) , a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies^[1].
Solvent and concentration: DMSO: 2 mM

指南 (以下是我们推荐的方案，本方案仅提供指南，应根据您的具体需要进行修改)。
1. 制备 Aβ 肽原纤维。
2. 将 N-Methylmesoporphyrin IX (NMM) (10 μM) 与 Aβ 肽 (50 μM) 在 37°C 下共孵育 7 天。
3. 在进行荧光测量前，额外添加 N-Methylmesoporphyrin IX 以维持其浓度恒定 (1 μM)。
4. 使用荧光显微镜或流式细胞仪进行观察 (Ex/Em = 399/610 nm)。

N-Methylmesoporphyrin IX (NMM) 对 G-四链体 DNA 敏感，但对双链体、三链体和单链 DNA 无响应。NMM 与四链体 DNA 结合后，为了实现有效的 π-π 堆积，可以调整其大环几何结构以匹配 G-四链体的末端，从而增强其荧光强度^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1161.46

C₇₀H₈₀N₈O₈

142234-85-3

液体（密度：1.261±0.14 g/cm³）

Dark purple to black

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Meng Li, et al. N-Methyl Mesoporphyrin IX as an Effective Probe for Monitoring Alzheimer's Disease β-Amyloid Aggregation in Living Cells. ACS Chem Neurosci. 2017 Jun 21;8(6):1299-1304.  [Content Brief]