2. NF-κB Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease
  3. NF-κB NO Synthase Interleukin Related TNF Receptor Cytochrome P450
  4. N,N-Diethylacetamide

N,N-Diethylacetamide 是一种极性溶剂，广泛用于薄膜、纤维制造及实验室中作为水不溶性化学品的载体。N,N-Diethylacetamide 通过抑制 NF-κB 通路发挥强效抗炎效果，抑制 NO、iNOS 表达，下调 TNF-α、IL-6 等关键炎症因子，但不影响 MAPK 通路。N,N-Diethylacetamide 可用于研究炎症性早产。

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N,N-Diethylacetamide

N,N-Diethylacetamide Chemical Structure

CAS No. : 685-91-6

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规格 价格 是否有货 数量
100 mg ￥100
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500 mg ￥180
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1 g ￥312
In-stock
5 g ￥562
In-stock
10 g ￥806
In-stock
50 g   询价  

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N,N-Diethylacetamide 相关抗体

Top Publications Citing Use of Products

查看 NF-κB 亚型特异性产品:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

查看 NO Synthase 亚型特异性产品:

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nNOS iNOS eNOS

查看 Interleukin Related 亚型特异性产品:

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

查看 Cytochrome P450 亚型特异性产品:

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CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

N,N-Diethylacetamide is a polar solvent widely used in film and fiber manufacturing, as well as in laboratories as a carrier for water-insoluble chemicals. N,N-Diethylacetamide exerts potent anti-inflammatory effects by inhibiting the NF-κB pathway, suppressing the expression of NO and iNOS, and downregulating key inflammatory cytokines such as TNF-α and IL-6, without affecting the MAPK pathway. N,N-Diethylacetamide can be used to study inflammatory preterm birth.

IC50 & Target

NF-κB

 

IL-1β

 

IL-6

 

IL-8

 

CYP2B1

 

CYP2B2

 

体外研究
(In Vitro)

N,N-Diethylacetamide (10 mM；26 h) 可抑制 RAW 264.7 细胞中 LPS (HY-D1056) 诱导的 NO 分泌[1]。
N,N-Diethylacetamide (10 mM；8 h) 可降低 RAW 264.7 细胞中 LPS 诱导的 iNOS 表达[1]。
N,N-Diethylacetamide (0.1-10 mM; 26 h) 可抑制 LPS 诱导的 HTR-8/SVneo 细胞中 IL-6、IL-8 和 MCP-1 的分泌[1]。
N,N-Diethylacetamide (10 mM; 26 h) 可抑制 RAW 264.7 细胞中 LPS 诱导的 TNF-α、IL-6、IL-1β、GM-CSF、MCP-1 及 IL-10 的分泌[1]。
N,N-Diethylacetamide (0.1-10 mM; 22 h) 可抑制 LPS 诱导的人胎盘组织块中细胞因子与趋化因子的分泌[1]。
N,N-Diethylacetamide (10 mM; 2.25 h) 可抑制 RAW 264.7 细胞中 LPS 诱导的 IkB-α 降解[1]。
N,N-Diethylacetamide (10 mM; 26 h) 不会改变 RAW 264.7 细胞中 LPS 诱导的 MAPK 通路蛋白表达[1]。
N,N-Diethylacetamide (10 mM; 26 h) 可在过表达 TLR4 的 HEK 293 细胞中抑制 LPS 诱导的 NF-κB 转录活性，且不影响 AP-1 或 C/EBP 的活性[1]。
N,N-Diethylacetamide (0.5-10 mM; 50 min) 可被纯化的 P4502B1 催化脱乙基化，其 Km 为 3.5 mM[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

N,N-Diethylacetamide 相关抗体:

Cell Viability Assay[1]

Cell Line: HTR-8/SVneo, RAW 264.7, HEK 293
Concentration: up to 10 mM
Incubation Time: 2 h (pre-treatment); 24 h (LPS treatment)
Result: Did not significantly affect the viability of HTR-8/SVneo, RAW 264.7, or HEK 293 cells compared to untreated controls.

Western Blot Analysis[1]

Cell Line: RAW 264.7
Concentration: 0.1, 1, 10 mM
Incubation Time: 2 h (pre-treatment); 6 h (LPS treatment)
Result: Significantly inhibited LPS-induced iNOS expression in RAW 264.7 cells at 10 mM (P < 0.001) compared to LPS control.

ELISA Assay[1]

Cell Line: HTR-8/SVneo
Concentration: 0.1, 1, 10 mM
Incubation Time: 2 h (pre-treatment); 24 h (LPS treatment)
Result: Significantly suppressed LPS-stimulated IL-6 secretion at 10 mM (more effectively than BAY 11-7082), caused a 90% reduction in IL-8 secretion at 10 mM (more effective than BAY 11-7082), and significantly inhibited MCP-1 secretion at 1 and 10 mM.

ELISA Assay[1]

Cell Line: RAW 264.7
Concentration: 0.1, 1, 10 mM
Incubation Time: 2 h (pre-treatment); 24 h (LPS treatment)
Result: Significantly reduced TNF-α (P < 0.0001), IL-6, IL-1β, GM-CSF (>90% reduction), MCP-1, and IL-10 (P < 0.0001) secretion from LPS-stimulated RAW 264.7 cells at 10 mM.

Western Blot Analysis[1]

Cell Line: RAW 264.7
Concentration: 0.1, 1, 10 mM
Incubation Time: 2 h (pre-treatment); 15 min (LPS treatment)
Result: Significantly inhibited LPS-induced IkB-α degradation in RAW 264.7 cells at 10 mM (P < 0.01) compared to LPS control.\nDid not affect LPS-stimulated expression of native or phosphorylated JNK1, ERK1/2, or p38 MAPK in RAW 264.7 cells at any concentration.
体内研究
(In Vivo)

N,N-Diethylacetamide (375-750 mg/kg；腹腔注射；两次给药) 可呈剂量依赖性延迟 LPS 诱导的早产，其中 750 mg/kg 剂量可完全阻止分娩至少 25 小时[1]。
N,N-Diethylacetamide (150-500 mg/kg；腹腔注射；每日一次；连续 3 天) 对大鼠给药可诱导 P4502B1/2 表达，并以剂量依赖的方式改变肝脏药物代谢酶活性，高剂量会引发肝毒性[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57Bl/6 (timed pregnant, weight 27–36 g)[1]
Dosage: 375 mg/kg; 750 mg/kg
Administration: i.p.; two doses (T=-15 min and T=10 h)
Result: Prevented delivery in all 6 mice treated with 750 mg/kg by 25 h post-LPS injection.
Delayed mean delivery time by approximately two hours and prevented delivery in 2/8 mice treated with 375 mg/kg by 25 h post-LPS injection.
Animal Model: Sprague-Dawley (male, 5-6 weeks old)[2]
Dosage: 150, 300 mg/kg; 400-500 mg/kg
Administration: i.p.; daily; 3 days
Result: Significantly induced the enzymatic activities related to CYP2B1/2 (such as PROD, 16β-testosterone hydroxylation), and enhanced its own metabolism (DEAC deethylase activity).
Increased the expression of CYP2B1/2 protein, while inhibiting the expression of CYP2C11.
分子量

115.17

Formula

C6H13NO
CAS 号
性状

液体（密度：0.925 g/cm3）

中文名称

二乙基乙酰胺
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
纯度 & 产品资料
参考文献

N,N-Diethylacetamide 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N,N-Diethylacetamide685-91-6二乙基乙酰胺NF-κBNO SynthaseInterleukin RelatedTNF ReceptorCytochrome P450Nuclear factor-κBNuclear factor-kappaBNitric oxide synthasesNOSILTumor Necrosis Factor ReceptorTNFRCYPsNF-κB pathwaycytochrome P450 2C11HTR-8/SVneocytochrome P450 2B1/2ratsinducible nitric oxide synthase (iNOS)MAPK pathwayRAW 264.7HEK 293LPSInhibitorinhibitorinhibit

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