R-PE (R-Phycoerythrin)  (Synonyms: 藻红蛋白)

R-Phycoerythrin is found in Heterosiphonia japonica. R-Phycoerythrin is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex: 495 nm).

R-Phycoerythrin (10-100 μM, 4 h) 以浓度依赖性方式对 S180 有细胞毒性 (58%)^[2]。
具体步骤^[2]：
1. 细胞处理与染色
1) S180 和 SMC7721 癌细胞在含 10% 胎牛血清和 1% 青/链霉素的 RPMI1640 培养基中培养至对数生长期 (37°C，5% CO₂，95% 空气湿度)。
2) 将细胞以约 5 × 10⁵ /mL 的密度接种于不含血清的 RPMI1640 培养基，加入不同浓度的 R-Phycoerythrin (10, 25, 50, 100 μM) 孵育 4 小时。
2. 光照处理
1) 使用 496 nm 氩激光激发 R-Phycoerythrin、β 亚基、γ 亚基，使用 514 nm 激发 α 亚基。
2) 换用含 10% 血清的 RPMI1640 培养基继续孵育 18 小时。
3) 使用 MTT 法检测 S180 细胞存活率。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

11016-17-4

液体

Brown to reddish brown

578

496

藻红蛋白

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Sun L, et al. Isolation, purification and characteristics of R-phycoerythrin from a marine macroalga Heterosiphonia japonica. Protein Expr Purif. 2009 Apr;64(2):146-54.  [Content Brief]

  [2]. Huang B, et al. The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT. Cancer Biother Radiopharm. 2002 Feb;17(1):35-42.  [Content Brief]