CFTR 蛋白, Human (HEK293)

CFTR Protein, Human (HEK293) is the recombinant human-derived CFTR, expressed by HEK293 , with tag Free labeled tag.

CFTR 是一种上皮离子通道，在调节上皮离子和水转运以及体液稳态中发挥重要作用。它介导氯离子跨细胞膜转运，并具有内在的 ATPase 活性，利用 ATP 来门控其通道功能；阴离子的被动流动通过 ATP 结合域的结合和水解循环来门控。该离子通道还对 HCO3- 具有通透性，选择性取决于细胞外氯离子浓度。在体外，CFTR 介导 ATP 依赖性谷胱甘肽通量，并通过调节其他离子通道和转运蛋白的活性发挥作用。它在气道液体稳态中起关键作用，调节气道表面液体层的 pH 值和离子含量，从而在病原体防御中发挥重要作用。CFTR 还通过调节 ENaC 复合体的细胞表面表达来调节其活性，并可能通过调节 SLC4A7 转运蛋白来调节上皮细胞的碳酸氢盐分泌和回收。此外，CFTR 可以抑制 ANO1 的氯通道活性，并在精子附睾成熟和获能过程中参与氯离子和碳酸氢盐稳态的调节。

Human

HEK293

Tag Free

P13569-1 (M1-L1480)

1080

Full Length of Isoform-1

Approximately 150 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl (pH 7.5), 2 mM MgCl2, 2 mM DTT, 200 mM NaCl, 0.005% (w/v) GDN, 0.0005% (w/v) CHS, Protease inhibitor.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.