GDF-5 蛋白, Mouse

GDF-5 protein is critical in bone and cartilage formation and promotes chondrogenic differentiation through dual pathways. It activates SMAD protein signaling and actively regulates chondrogenesis through high-affinity binding to BMPR1B and low-affinity binding to BMPR1A. GDF-5 Protein, Mouse is the recombinant mouse-derived GDF-5 protein, expressed by E. coli , with tag free.

GDF-5 蛋白在骨和软骨形成中发挥着至关重要的作用，通过双重途径协调软骨组织的分化。它通过与 BMPR1B 高亲和力结合和与 BMPR1A 较低亲和力结合，触发 SMAD1-SMAD5-SMAD8 复合物的磷酸化和随后的 SMAD 蛋白信号转导，从而正向调节软骨形成分化。同时，它通过与NOG相互作用对软骨形成分化发挥负调节作用。此外，GDF-5 对于防止去神经支配后肌肉过度流失至关重要，这是一种由磷酸化 SMAD1/5/8 介导的功能。该蛋白还结合细菌脂多糖 (LPS) 并介导 LPS 诱导的炎症反应，包括单核细胞分泌 TNF。在结构上，GDF-5 形成通过二硫键连接的同二聚体，并与丝氨酸蛋白酶 HTRA1 和 HTRA3 相互作用。LPS 结合后，它还可能与 CXCR4、HSP90AA1 和 HSPA8 形成复合物。此外，GDF-5 与 NOG 高亲和力相互作用，抑制软骨形成，并与 BMPR1B 和 BMPR1A 相互作用，通过 SMAD 依赖性信号传导正向调节软骨细胞分化。此外，它还与 FBN1 和 FBN2 相互作用。

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.2-1.2 µg/mL.

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  Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.4147 µg/mL, corresponding to a specific activity is 2.411×10^3 units/mg.

Mouse

E. coli

Tag Free

P43027 (A376-R495)

14563  [NCBI]

Full Length of Growth/differentiation factor 5

Approximately 16 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.