IFN-beta 蛋白, Rhesus Macaque (HEK293, Fc, Solution)

IFN-beta Protein, Rhesus Macaque (HEK293, Fc, Solution) is the recombinant rhesus macaque-derived IFN-beta protein, expressed by HEK293, with C-mFc tag.

IFN-β 蛋白是一种 I 型干扰素细胞因子，在针对感染、肿瘤和各种炎症刺激的先天免疫反应中发挥着关键作用。其信号传导涉及与高亲和力受体 IFNAR2 和低亲和力受体 IFNAR1 结合，形成异二聚体复合物并激活经典的 Jak-STAT 信号传导途径。这种激活导致干扰素调节基因的转录调节，包括抗病毒蛋白、细胞增殖和分化的调节因子以及免疫调节蛋白。虽然主要通过 IFNAR1-IFNAR2 异二聚体受体发出信号，但 IFN-β 也可以单独与 IFNAR1 一起发挥作用，独立于 Jak-STAT 途径发挥作用。IFN-β 引发多种反应，包括抗病毒和抗菌活性，并影响 B 细胞发育、骨髓生成和脂多糖 (LPS) 诱导的肿瘤坏死因子的产生。除了其免疫功能外，IFN-β 通过调节多巴胺更新和保护多巴胺能神经元、促进神经元自噬、促进 α-突触核蛋白清除，从而防止多巴胺能神经元丢失，在神经元稳态中发挥着至关重要的作用。值得注意的是，IFN-β 在诱导细胞凋亡和抗增殖途径方面比干扰素 α (IFN-α) 表现出更强的效力，这对控制肿瘤细胞生长至关重要。它在这些监管过程中充当单体。

Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED₅₀ for this effect is 0.1-0.5 ng/mL.

Rhesus Macaque

HEK293

C-mFc

EHH24077.1 (M22-N187)

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Full Length of Mature Protein

MSYNLLGFLQRSSSFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQPQQFQKEDAALTIYEMLQNIFAIFRQDLSSTGWNETIVENLLANVYHQIDHLKTILEEKLEKEDFTRGKFVSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFFFINKLTGYLRN

Approximately 40-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.