UBE2C 蛋白, Human (sf9, His, StrepⅡ)

The UBE2C protein is a key ubiquitin-proteasome system component that acts as an E2 ubiquitin-conjugating enzyme and is the core of the covalent linkage of ubiquitin to target proteins. In vitro, UBE2C expertly promotes “Lys-11” and “Lys-48” linked polyubiquitination, demonstrating the versatility of ubiquitin chain formation. UBE2C Protein, Human (sf9, His, Strep) is the recombinant human-derived UBE2C protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

UBE2C 是泛素-蛋白酶体系统的关键组成部分，作为 E2 泛素结合酶发挥作用，在泛素与靶蛋白的共价连接中发挥核心作用。在体外，UBE2C 在促进“Lys-11”和“Lys-48”连接的多泛素化方面表现出催化能力，展示了其在泛素链形成中的多功能性。至关重要的是，UBE2C 是后期促进复合物/环体 (APC/C) 中不可或缺的因子，APC/C 是一种细胞周期调节的泛素连接酶，对于控制有丝分裂进展至关重要。它在 APC/C 底物上启动“Lys-11”连接的多聚泛素链中的作用强调了其在协调蛋白酶体降解特定蛋白质方面的重要性，从而促进有丝分裂的及时和受控退出。

The ubiquitin conjugating activity of UBE2C was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme UBE1 and Bodipyubiquitin. Incubation of UBE2C for 60 minutes at 37˚C in the presence of Bodipy-ubiquitin, UBE1 and ATP was compared alongside two control reactions with either ATP or UBE2C excluded from the reaction. Ubiquitin conjugates were identified by the migration of the Bodipy-ubiquitin band and these were observed only in the presence of ATP and UBE2C.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

O00762 (A2-P179)

11065

Full Length

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

1.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.