WRN 蛋白, Human (His)

WRN Protein, Human (His) is the recombinant human-derived WRN protein, expressed by E. coli, with N-His tag.

WRN 是一种多功能酶，具有镁和 ATP 依赖性的 3'-5' DNA 解旋酶活性，作用于部分双链底物。它还对具有 5' 突出端的双链 DNA (dsDNA) 表现出 3'->5' 外切酶活性，但对单链 DNA (ssDNA) 或平末端 dsDNA 无核酸酶活性。解旋酶活性在 (d)ATP 存在时效率最高，(d)CTP 可替代但效率降低；单链结合蛋白 (异源三聚体复制蛋白 A 复合物，RPA1、RPA2、RPA3) 可增强链置换。WRN 优先结合含有交替二级结构的 DNA 底物，如复制叉和 Holliday 连接体，可能在解离同源重组产物、停滞复制叉或 DNA 修复过程中产生的联合 DNA 分子中起重要作用。它还能缓解 DNA 聚合酶在 DNA 损伤位点的停滞，并在 DNA 复制焦点中心形成中发挥作用；与 foci 元件稳定结合，为 RP-A 生成结合位点 (by similar)。WRN 参与 γ 辐射后的双链断裂修复，可解旋某些 G-四链体 DNA (d(CGG)n 片段)，解旋似乎以 5'-3' 和 3'-5' 双向进行并需要短单链尾端。d(CGG)n 片段易于组装成四链体结构；来自端粒或 IgG 转换序列的其他 G 富集底物不被解旋。WRN 缺失会导致染色体断裂和基因组不稳定。

Human

E. coli

N-His

Q14191 (S2-I333)

7486

Partial

SEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVRKSVFEDDLPFLEFTGSIVYSYDASDCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFEDSTTGGVQQKQI

Approximately 40-43 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.