Rhod-2 AM (solution) 

Rhod-2 (solution) is a high-affinity visible light excitation wavelength Ca²⁺ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm^[1].
Solvent and concentration: DMSO: 1 mM

1. Rhod-2 AM工作液的配制
1.1 Rhod-2 AM工作液的配制
用预热好的无血清细胞培养基或 PBS 稀释储存液，可根据实际情况稀释相应的储备液。注意，如果溶剂为 DMSO，则必须考虑 DMSO 的细胞毒性，并应制备溶剂对照；如果溶剂为纯水，则工作液在加入细胞前需要过滤并除菌。
注：请根据实际情况调整 Rhod-2 AM 工作液的浓度。
2. 细胞染色 (6孔板) 2.1 悬浮细胞 a. 4°C、1000g 离心 3-5 分钟，弃上清。PBS 洗两次，每次 5 分钟。细胞密度为 1×10⁶/mL。
b.加入1 mL 工作液，然后室温孵育 5-30 分钟。
c. 4°C、400g 离心 3-4 分钟，弃上清。
d. 用 PBS 洗涤两次，每次 5 分钟。
e. 用无血清细胞培养基或 PBS 重悬细胞。 通过荧光显微镜或流式细胞术观察。
2.2 贴壁细胞
a. 在无菌盖玻片上培养贴壁细胞。
b. 从培养基中取出盖玻片并吸出多余的培养基。
c. 加入100 μL 工作液，轻轻摇匀，使细胞完全覆盖，室温孵育 5-30 分钟。
d. 用培养基洗涤两次，每次 5 分钟。荧光显微镜或流式细胞仪观察。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1123.96

C₅₂H₅₉BrN₄O₁₉

145037-81-6

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35.  [Content Brief]

  [2]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35.  [Content Brief]