Temporin-SHa 

Temporin-Sha is an antibacterial peptide with extensive biological activity. Temporin-Sha exhibits broad-spectrum antibacterial activity (e.g., against L. ivanovii, MIC = 6.25 μM), and is effective against Gram-negative bacteria (such as Escherichia coli, MIC = 10 μM), including drug-resistant strains (such as Methicillin (HY-121544)-resistant Staphylococcus aureus). Temporin-Sha also has inhibitory effects on Candida albicans (MIC = 25 μM), Saccharomyces cerevisiae (MIC = 12 μM), the pre-flagellated and non-flagellated forms of Leishmania infantum (IC₅₀ = 5-20 μM), and Trypanosoma cruzi (IC₅₀ = 17 μM). Temporin-Sha exhibits antiviral activity against HSV-1 and has anti-cancer effects (cytotoxicity against breast cancer cells MCF-7 and lung cancer cells H460, etc.)^{[1][2][3][4][5]}.

Temporin-Sha (12.5 μM, 3 h) 可导致伊氏李斯特菌 (Listeria ivanovii) 的菌膜破裂，即使嫁接到金表面后，仍可杀死 75% 以上的细菌^[1]。
Temporin-Sha (2-1024 μg/mL, 0-24 h) 对 Fluconazole (HY-B0101) 敏感 (CaS) 和 Fluconazole 耐药 (CaR) 白色念珠菌 (MIC ≥ 256 μg/mL) 具有显著的抑菌效果，并可抑制 CaS 和 CaR 生物膜的形成，在高浓度 (1024 μg/mL) 下仍具有破坏已形成的生物膜的能力^[2]。
Temporin-Sha (32-1024 μg/mL) 在所有浓度下均对正常口腔角质形成细胞 (NOK-si) (CC₅₀ = 3805 μg/mL) 无细胞毒性，对人牙龈成纤维细胞 (FGH) (CC₅₀ = 492 μg/mL) 表现出轻微细胞毒性^[2]。
Temporin-Sha (1.25-10 μM，25 h) 通过直接破坏病毒颗粒 (不依赖免疫调节) 显著抑制人类原代角质形成细胞中的 HSV-1 DNA 复制^[3]。
Temporin-Sha (2-50 μM，0-180 min) 可有效改变细菌 (大肠杆菌、化脓性链球菌、肺炎克雷伯菌、乳酸杆菌) 和寄生虫 (T. cruzi) 质膜的完整性，并诱导 Leishmania infantum 前鞭毛体发生凋亡样死亡^[4]。
Temporin-Sha (0-60 d) 在大肠杆菌中连续培养 55 代后，与 Ampicillin (HY-B0522) 不同，未表现出促进细菌耐药性的倾向^[4]。
Temporin-SHa 对多种癌细胞系具有细胞毒性，IC₅₀ 值分别为 14.47 μM (MCF-7)、18.36 μM (HeLa) 和 34.5 μM (NCI-H460)，诱导的细胞死亡主要是坏死而非凋亡^[5]。
Temporin-SHa 对多种癌细胞系具有细胞毒性，IC₅₀ 值分别为 14.47 μM (MCF-7)、18.36 μM (HeLa) 和 34.5 μM (NCI-H460)，并且诱导的细胞死亡主要是坏死而非凋亡^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1380.74

C₆₇H₁₀₉N₁₅O₁₄S

1065010-73-2

Phe-Leu-Ser-Gly-Ile-Val-Gly-Met-Leu-Gly-Lys-Leu-Phe-NH2

FLSGIVGMLGKLF-NH2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lombana A, et al. Temporin-SHa peptides grafted on gold surfaces display antibacterial activity. J Pept Sci. 2014 Jul;20(7):563-9.  [Content Brief]

  [2]. Dias LM, et al. Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans. Microorganisms. 2024 Jan 4;12(1):99.  [Content Brief]

  [3]. Roy M, et al. Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K³]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1. Viruses. 2019 Jan 18;11(1):77.  [Content Brief]

  [4]. Raja Z, et al. Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent. PLoS One. 2017 Mar 20;12(3):e0174024.  [Content Brief]

  [5]. Shaheen F, et aI. Synthesis of breast cancer targeting conjugate of temporin-SHa analog and its effect on pro- and anti-apoptotic protein expression in MCF-7 cells. Peptides. 2018 Aug;106:68-82.  [Content Brief]