Texas Red (solution) 

Texas Red (Sulforhodamine 101) (solution) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations^[1].
Solvent and concentration: DMSO: 10 mM

说明：Texas Red (Sulforhodamine 101) 可作为星形胶质细胞标记物，用于海马切片的红色荧光染色^[3]。 方法：用于细胞染色。
1. 将海马切片置于含碳人工脑脊液 (ACSF) 中，用 Texas Red (5 μM；35 分钟；34°C) 染色。
2. 将海马切片放回无染料的 ACSF 中至少 30 分钟，再进行成像。
3. 使用共聚焦激光扫描显微镜 (Zeiss LSM 880) 进行成像。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

在体实验中，通过海马内微量注射10 μM Texas Red 或局部施用100 μM Texas Red 均可诱发癫痫样电活动^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

606.71

C₃₁H₃₀N₂O₇S₂

60311-02-6

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Axel Nimmerjahn, et al. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2004 Oct;1(1):31-7.  [Content Brief]

  [2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9.  [Content Brief]

  [3]. Wang T, et al. Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. Neuropharmacology. 2022 Oct 1;217:109191.  [Content Brief]