2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Apoptosis
  3. Topoisomerase Reactive Oxygen Species (ROS) Apoptosis MDM-2/p53 Caspase Bcl-2 Family
  4. Topo I/II-IN-3

Topo I/II-IN-3 是一种拓扑异构酶 I/II (topoisomerase I/II) 双重抑制剂，Topo I 的 IC50 为 8.99 μM，Topo II 的 IC50 为 26.92 μM。Topo I/II-IN-3 可诱导 DNA 损伤、升高细胞内的 ROS 水平、激活线粒体凋亡 (apoptosis) 通路、诱导癌细胞产生细胞毒性。Topo I/II-IN-3 上调细胞中 γ-H2AX 的表达，上调 p53、活化的 caspase-9、Bax 及活化的 caspase-3，同时下调 Bcl-2 的表达。Topo I/II-IN-3 可用于乳腺癌、肝癌、胃癌的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Topo I/II-IN-3

Topo I/II-IN-3 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

Topo I/II-IN-3 相关抗体

Top Publications Citing Use of Products

查看 Topoisomerase 亚型特异性产品:

查看所有亚型
Topoisomerase Topo I Topo II DNA gyrase

查看 Caspase 亚型特异性产品:

查看所有亚型
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

查看 Bcl-2 Family 亚型特异性产品:

查看所有亚型
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Topo I/II-IN-3 is a dual inhibitor of topoisomerase I/II (topoisomerase I/II), with an IC50 of 8.99 μM against Topo I and an IC50 of 26.92 μM against Topo II. Topo I/II-IN-3 induces DNA damage, elevates intracellular ROS levels, activates the mitochondrial apoptosis pathway, and exerts cytotoxicity against cancer cells. Topo I/II-IN-3 upregulates the expression of γ-H2AX, p53, activated caspase-9, Bax and activated caspase-3, while downregulating the expression of Bcl-2. Topo I/II-IN-3 can be used in research related to breast cancer, liver cancer and gastric cancer[1].

IC50 & Target[1]

Topoisomerase I

8.99 ()

Topoisomerase II

26.92 ()

Caspase-9

 

Caspase-3

 

Bax

 

体外研究
(In Vitro)

Topo I/II-IN-3 (Compound A6) (0.6-22.1 μM; 48 h) 可强效抑制 MCF-7、MDA-MB-231、HepG2 和 SGC-7901 癌细胞的增殖，IC50 分别为 0.6、12.6、3.4、5.4 μM，且对正常 HEK-293 细胞的细胞毒性较低 (IC50 = 22.1 μM)[1]。
Topo I/II-IN-3 (1.56-100 μM; 30 min) 可阻断 DNA 松弛作用和 kDNA 解连环作用，IC50 分别为为 8.99、26.92 μM[1]。
Topo I/II-IN-3 (0.3-1.2 μM; 24 h) 可在 MCF-7 细胞中诱导剂量依赖性 DNA 损伤，上调细胞中 γ-H2AX 的表达，上调 p53、活化的 caspase-9、Bax 及活化的 caspase-3，同时下调 Bcl-2[1]。
Topo I/II-IN-3 (0.25-1 μM; 24-48 h) 可强效抑制 MCF-7 细胞的长期增殖，抑制 MCF-7 细胞的迁移[1]。
Topo I/II-IN-3 (0.3-1.2 μM; 24 h) 可在 MCF-7 细胞中诱导剂量依赖性的 G2/M 期阻滞，诱导细胞凋亡，升高细胞内的 ROS 水平，降低线粒体膜电位[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Topo I/II-IN-3 相关抗体:

Western Blot Analysis[1]

Cell Line: MCF-7
Concentration: 0, 0.3, 0.6, 1.2 μM
Incubation Time: 24 h
Result: Markedly upregulated γ-H2AX protein expression in a dose-dependent manner, confirming induction of DNA double-strand breaks.\nDose-dependently upregulated expression of p53, cleaved caspase-9, Bax, and cleaved caspase-3, while downregulating Bcl-2 expression.

Cell Proliferation Assay[1]

Cell Line: MCF-7
Concentration: 0, 0.25, 0.5, 1 μM
Incubation Time: 48 h
Result: Significantly impaired clonogenicity in a dose-dependent manner; at 1 μM, colony formation fell to below 20% of the control.

Cell Migration Assay [1]

Cell Line: MCF-7
Concentration: 0, 0.25, 0.5, 1 μM
Incubation Time: 24 h, 48 h
Result: Significantly reduced cell migration in a dose-dependent manner; at 1 μM, anti-migratory effects were comparable to positive controls CPT and VP-16.

Cell Cycle Analysis[1]

Cell Line: MCF-7
Concentration: 0, 0.3, 0.6, 1.2 μM
Incubation Time: 24 h
Result: Induced dose-dependent G2/M phase arrest, with increasing proportions of cells in G2/M phase and decreasing proportions in G0/G1 phase as concentrations rose.

Apoptosis Analysis[1]

Cell Line: MCF-7
Concentration: 0, 0.3, 0.6, 1.2 μM
Incubation Time: 24 h
Result: Significantly induced apoptosis in a dose-dependent manner; at 1.2 μM, the total apoptosis rate reached approximately 40%, comparable to positive controls.
分子量

414.56

Formula

C23H35FN6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Topo I/II-IN-3 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Topo I/II-IN-3TopoisomeraseReactive Oxygen Species (ROS)ApoptosisMDM-2/p53CaspaseBcl-2 Familyhuman Topo IIbreast cancerMCF-7liver cancerMDA-MB-231gastric cancertopoisomerase Itopoisomerase IIHepG2human Topo IInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Topo I/II-IN-3
目录号：
HY-183330
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z