TPE-MI (solution) 

TPE-MI (Tetraphenylethene maleimide) (solution) is a thiol probe for measuring unfolded protein load and proteostasis in cells (the excitation wavelength is 350 nm and the emission wavelength is 470 nm). TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin research of the malaria parasitesPlasmodium falciparum ^[1][2].
Solvent and concentration: DMSO: 10 mM

指南 (以下是我们推荐的方案，本方案仅提供指南，应根据您的具体需要进行修改)。
1 染料储备液^[1]
TPE-MI 储存液建议分装后于 -20°C 或 -80°C 避光保存。
2 染料工作液制备
1) 使用前，用 PBS 将 TPE-MI 储备液稀释至工作浓度。可根据实际情况稀释相应的储备液。注意，如果溶剂为 DMSO，则必须考虑 DMSO 的细胞毒性，并应制备溶剂对照；如果溶剂为纯水，则工作液在加入细胞前需要过滤并除菌。
注:请根据实际情况调整 TPE-MI 工作液浓度，且现用现配。
3 具体染色步骤
1) 细胞培养准备：将细胞 (如 HeLa、Neuro2a 细胞) 接种于合适的培养容器 (如 8 孔 μ-slides) 中。
2) 清洗：用 PBS 洗涤细胞，去除培养基。
3) 染色：向细胞中加入 50 μM 的 TPE-MI 工作液，于 37 °C 孵育 30 min。
4) 染色后处理：
流式细胞术检测：移除 TPE-MI 溶液，用合适方法 (如胰酶消化、Accutase) 消化细胞，用 PBS 重悬后离心 (120 g，6 min) ，弃上清，再用 PBS 重悬细胞用于分析。
SDS-PAGE 检测：用细胞裂解液裂解染色后的细胞，测定蛋白浓度，经 12% 聚丙烯酰胺 SDS-PAGE 电泳后，进行荧光和考马斯亮蓝染色分析。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

441.53

C₃₁H₂₃NO₂

1245606-71-6

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chen MZ, et al. A thiol probe for measuring unfolded protein load and proteostasis in cells. Nat Commun. 2017;8(1):474. Published 2017 Sep 7.  [Content Brief]

  [2]. Hu Q, et al. In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe. Molecules. 2021;26(14):4273. Published 2021 Jul 14.  [Content Brief]