2. Neuronal Signaling
  3. Amyloid-β
  4. β-Amyloid (25-35), HFIP-treated

β-Amyloid (25-35), HFIP-treated  (Synonyms: Amyloid beta-peptide (25-35), HFIP-treated; Aβ25-35, HFIP-treated; β-Amyloid peptide (25-35), HFIP-treated)

目录号: HY-P0128A 纯度: 99.71%
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β-Amyloid (25-35) (Amyloid beta-peptide (25-35)), HFIP-treated 是经过 HFIP 处理后的 β-Amyloid (25-35) (HY-P0128)。β-Amyloid (25-35) 是阿尔茨海默氏淀粉样蛋白 β 肽的 Aβ(25-35) 片段，在培养细胞中显示出神经毒性活性。

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Custom Peptide Synthesis

β-Amyloid (25-35), HFIP-treated

β-Amyloid (25-35), HFIP-treated Chemical Structure

CAS No. : 131602-53-4

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1 mg ￥1600
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1 mg * 5 ￥4000
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Other Forms of β-Amyloid (25-35), HFIP-treated:

β-Amyloid (25-35), HFIP-treated 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 32 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)), HFIP-treated is a β-Amyloid (25-35) (HY-P0128) treated with HFIP. β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells[1].

体外研究
(In Vitro)

Aβ(25-35) 肽的氨基酸序列为 NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH，其中第一个 Gly 代表氨基酸 25，最后一个 Met 代表氨基酸 35。还首次在凝胶状态下研究了淀粉样 β 肽 (25-35)。还利用振动吸收和 ECD 进行了比较研究。还利用振动吸收和 VCD 光谱研究了 Aβ(25-35) 肽膜的构象偏好[1]。
Aβ(25-35) 肽可诱导分离的脑线粒体凋亡和蛋氨酸-35 的氧化还原状态，在诱导程序性细胞死亡途径和毒性事件中起关键作用[2]。
β-淀粉样蛋白聚集指南（以下是我们推荐的方案。该方案仅提供指南，应根据您的具体需求进行修改）。
1. 将所得膜溶解于无水 DMSO 中，浓度为 5 mM，然后涡旋稀释至适当浓度和缓冲液（无血清和无酚红培养基）中。
2. 接下来，将溶液在 37°C 下陈化 7 天。然后将样品在 4-8 °C 下以 14000g 离心 10 分钟；可溶性低聚物在上清液中。将上清液稀释 10-200 倍用于实验。
方法因下游应用而异。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

β-Amyloid (25-35), HFIP-treated 相关抗体:
分子量

1060.27

Formula

C45H81N13O14S
CAS 号
性状

固液混合物

颜色

White to off-white

Sequence

Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met
Sequence Shortening

GSNKGAIIGLM
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

纯度 & 产品资料
参考文献
Cell Assay
[2]

Cell viability is determined by a modified MTS assay, which is based on the conversion of Tetrazolium salt by mitochondrial dehydrogenase to a formazan product spectrophotometrically measurable at 490 nm. PC12 cells are plated in 96-well plates at a density of 10 000 cells/well and maintained for 16 h in complete medium. Cells are then incubated in the absence (control) and presence of 40 μM Aβ(31-35) and Aβ(25-35) with reduced, oxidized and norleucine-substituted methionine-35 staurosporine 10 μM is used as positive control of 100% of cellular death. After 48 h of peptide-incubation, 20 μL of MTS reagent (2.0 mg/mL) is added to each well. The cells are then incubated for 30-45 min at 37 °C in a 5% CO2 incubator. The reaction is stopped by adding 25 μL of 10% SDS. The plates are read with a microplate reader at 490 nm. Each data point is obtained using a triplet-well assay[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

β-Amyloid (25-35), HFIP-treated 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Amyloid (25-35), HFIP-treated131602-53-4Amyloid beta-peptide (25-35), HFIP-treated Aβ25-35, HFIP-treatedβ-Amyloid peptide (25-35), HFIP-treatedAmyloid-ββ-amyloid peptideAβAbetaInhibitorinhibitorinhibit

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