HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL5257818

Protocol: N/A

Comment: Journal: Eur J Med Chem
Year: 2016
Volume: 123
First Page: 236
Last Page: 255
DOI: 10.1016/j.ejmech.2016.07.056

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units	Data Validity Comment
174	IC50	=	174000	nM	Outside typical range

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Arachidonate 5-lipoxygenase

External ID: CHEMBL3874972

Protocol: N/A

Comment: Journal: Bioorg Med Chem
Year: 2017
Volume: 25
Issue: 3
First Page: 1172
Last Page: 1182
DOI: 10.1016/j.bmc.2016.12.025

Target ChEMBL ID: CHEMBL3879862
ChEMBL Target Name: Arachidonate 5-lipoxygenase
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value
Ratio IC50	=	40

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type II subunit gamma

External ID: CHEMBL3430795

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL3829
ChEMBL Target Name: CaM kinase II gamma
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	-11.5	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	2.2	degrees C
Thermal melting change	=	2.9	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	4.9	degrees C
Thermal melting change	=	6.2	degrees C
Thermal melting change	=	5.2	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	4.5	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	0.4	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type IV

External ID: CHEMBL3430796

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2494
ChEMBL Target Name: CaM kinase IV
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	2.1	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	2.8	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	-0.8	degrees C
Thermal melting change	=	6.7	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	6	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	4.8	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	4.2	degrees C
Thermal melting change	=	0.9	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089625

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type II subunit beta

External ID: CHEMBL3430793

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4121
ChEMBL Target Name: CaM kinase II beta
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	2.9	degrees C
Thermal melting change	=	2.8	degrees C
Thermal melting change	=	2.2	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.5	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	1	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type II subunit delta

External ID: CHEMBL3430794

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2801
ChEMBL Target Name: CaM kinase II delta
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	1.9	degrees C
Thermal melting change	=	2.7	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	-0.5	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.6	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Cyclin-dependent kinase 6

External ID: CHEMBL3430799

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2508
ChEMBL Target Name: Cyclin-dependent kinase 6
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	1.9	degrees C
Thermal melting change	=	2	degrees C
Thermal melting change	=	2.1	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	-0.4	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089628

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Cyclin-dependent kinase-like 1

External ID: CHEMBL3430800

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL5789
ChEMBL Target Name: Cyclin-dependent kinase-like 1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	-0.5	degrees C
Thermal melting change	=	2.7	degrees C
Thermal melting change	=	-0.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Focal adhesion kinase 1

External ID: CHEMBL2089629

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL2695
ChEMBL Target Name: Focal adhesion kinase 1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Units	Activity Comment
Inhibition	%	Dose-dependent effect

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase kinase 2

External ID: CHEMBL3430797

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL5284
ChEMBL Target Name: CaM-kinase kinase beta
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	3	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	5.5	degrees C
Thermal melting change	=	7.9	degrees C
Thermal melting change	=	6.8	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	-0.5	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	-1.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089626

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Units	Activity Comment
Inhibition	%	Dose-dependent effect

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Cyclin-dependent kinase 2

External ID: CHEMBL3430798

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL301
ChEMBL Target Name: Cyclin-dependent kinase 2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	1.5	degrees C
Thermal melting change	=	1.7	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	5.3	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	-0.8	degrees C
Thermal melting change	=	-0.8	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	1.5	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	0.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089627

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Units	Activity Comment
Inhibition	%	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Dual specificity protein kinase CLK2

External ID: CHEMBL3430803

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4225
ChEMBL Target Name: Dual specificity protein kinase CLK2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	2.8	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	2.5	degrees C
Thermal melting change	=	4.1	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	4.9	degrees C
Thermal melting change	=	5.8	degrees C
Thermal melting change	=	5.9	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	3.6	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	4.5	degrees C
Thermal melting change	=	-0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Dual specificity protein kinase CLK3

External ID: CHEMBL3430804

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4226
ChEMBL Target Name: Dual specificity protein kinase CLK3
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	3.1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	2.5	degrees C
Thermal melting change	=	2.7	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	5.4	degrees C
Thermal melting change	=	2.2	degrees C
Thermal melting change	=	2.1	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	4.2	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	6.1	degrees C
Thermal melting change	=	-0.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Serine/threonine-protein kinase Chk2

External ID: CHEMBL3430801

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2527
ChEMBL Target Name: Serine/threonine-protein kinase Chk2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.5	degrees C
Thermal melting change	=	2.7	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	7.1	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	4.8	degrees C
Thermal melting change	=	10.3	degrees C
Thermal melting change	=	12.9	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	2	degrees C
Thermal melting change	=	14.1	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	7.5	degrees C
Thermal melting change	=	0.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：NCGC 靶标：DNA polymerase kappa [Homo sapiens]

External ID: PolK100

Protocol: Three microliters of reagents (buffer in column 3 and 4 as negative control and 10 nM Pol kappa in columns 1, 2, and 5-48) were dispensed into a 1536-well black solid-bottom plate. Compounds (23 nL) were transferred via Kalypsys pin tool equipped with 1536-pin array. The plates were then incubated for 15 min at room temperature, and 1 uL substrate (50 nM final concentration) were then added to start the reaction and kinetically read twice at 0 min and 10 min on the Viewlux reader

Comment: Compound Ranking:

1. Compounds are first classified as having full titration curves, partial modulation, partial curve (weaker actives), single point activity (at highest concentration only), or inactive. See data field "Curve Description". For this assay, apparent inhibitors are ranked higher than compounds that showed apparent activation.
2. For all inactive compounds, PUBCHEM_ACTIVITY_SCORE is 0. For all active compounds, a score range was given for each curve class type given above. Active compounds have PUBCHEM_ACTIVITY_SCORE between 40 and 100. Inconclusive compounds have PUBCHEM_ACTIVITY_SCORE between 1 and 39. Fit_LogAC50 was used for determining relative score and was scaled to each curve class' score range.

Phenotype	Fit_CurveClass	Excluded_Points	Max_Response	Activity at 0.477 uM	Activity at 2.393 uM	Activity at 11.99 uM	Activity at 60.11 uM	Activity at 107.2 uM	Compound QC
Inactive	4	0 0 0 0 0	1.4694	-3.5669	-6.235	2.8586	1.8042	1.4694	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	-4.2631	8.2218	8.0811	10.2927	-3.9947	-4.2631	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	6.0369	0.3398	-2.1048	-8.1695	-3.6822	6.0369	QC'd by "Chem Div"
Inactive	4		-2.0565	1.7294	-3.5894	-1.2575	-0.5402	-2.0565	QC'd by "Chem Div"
Inactive	4	0 0 0 0 1	2.3149	1.0048	4.6369	-1.9963	-3.3543	2.3149	QC'd by "Chem Div"
Inactive	4		7.2748	7.1515	6.1372	1.5197	5.2332	7.2748	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	1.006	-3.3873	-7.786	-9.3037	-9.1761	1.006	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	-0.0368	-9.4458	-10.5155	-9.0065	-12.9141	-0.0368	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	2.6	-7.8084	-12.3007	-2.0954	-6.6887	2.6	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	-11.4867	-18.9051	-17.4955	-19.0735	-9.6682	-11.4867	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	-7.5605	-17.2173	-11.0038	-16.5656	-22.4025	-7.5605	QC'd by "Chem Div"
Inactive	4		-7.5451	-1.1939	-1.3084	-5.8268	-5.3206	-7.5451	QC'd by "Chem Div"
Inactive	4	0 0 0 0 1	-5.5852	-4.3753	-1.0046	-3.1641	-10.1524	-5.5852	QC'd by "Chem Div"
Inactive	4	0 0 0 0 0	1.1172	-6.0391	7.0118	9.0446	1.6533	1.1172	QC'd by "Chem Div"
Inactive	4		2.3359	1.2518	1.6626	-0.9325	-0.9194	2.3359	QC'd by "Chem Div"
Inactive	4	0 0 0 0	-19.5354	0.3984	-4.1147	2.1883	-19.5354		QC'd by "Chem Div"
Inactive	4		-5.6552	-4.6769	-1.9378	-0.5867	-3.224	-5.6552	QC'd by "Chem Div"
Inactive	4		-11.3738	-10.4148	-13.8912	-10.4252	-7.8961	-11.3738	QC'd by "Chem Div"
Inactive	4		-6.1571	-8.7102	-2.9113	-5.2229	-3.4369	-6.1571	QC'd by "Chem Div"
Inactive	4	0 0 0 0 1	-7.3803	-8.8177	-11.1654	-6.5301	-15.9483	-7.3803	QC'd by "Chem Div"

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Dual specificity protein kinase CLK1

External ID: CHEMBL3430802

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4224
ChEMBL Target Name: Dual specificity protein kinase CLK1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	3.9	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	4.1	degrees C
Thermal melting change	=	6.3	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	7.7	degrees C
Thermal melting change	=	6.6	degrees C
Thermal melting change	=	5.1	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	2.1	degrees C
Thermal melting change	=	-0.5	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	-1.2	degrees C
Thermal melting change	=	6.6	degrees C
Thermal melting change	=	4.3	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	5.5	degrees C
Thermal melting change	=	0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Casein kinase I isoform gamma-3

External ID: CHEMBL3430807

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL5084
ChEMBL Target Name: Casein kinase I isoform gamma-3
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	2.9	degrees C
Thermal melting change	=	3.8	degrees C
Thermal melting change	=	4.9	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	0.4	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Death-associated protein kinase 3

External ID: CHEMBL3430808

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2468
ChEMBL Target Name: Death-associated protein kinase 3
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	3.5	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	2.8	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	7.5	degrees C
Thermal melting change	=	5.9	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	4.3	degrees C
Thermal melting change	=	11.8	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	3.4	degrees C
Thermal melting change	=	-0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Casein kinase I isoform gamma-1

External ID: CHEMBL3430805

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2426
ChEMBL Target Name: Casein kinase I gamma 1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	3.2	degrees C
Thermal melting change	=	4.3	degrees C
Thermal melting change	=	4.5	degrees C
Thermal melting change	=	3	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	3	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	2	degrees C
Thermal melting change	=	0.5	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Casein kinase I isoform gamma-2

External ID: CHEMBL3430806

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2543
ChEMBL Target Name: Casein kinase I gamma 2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	4	degrees C
Thermal melting change	=	4.9	degrees C
Thermal melting change	=	2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	1.7	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Solute carrier family 22 member 2

External ID: CHEMBL5229529

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2023
Volume: 86
Issue: 1.0
First Page: 191
Last Page: 198
DOI: 10.1021/acs.jnatprod.2c00927

Target ChEMBL ID: CHEMBL1743122
ChEMBL Target Name: Solute carrier family 22 member 2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units	Data Validity Comment
17.33	IC50	=	17330	nM
39.47	IC50	=	39470	nM
1617	IC50	=	1617000	nM	Outside typical range
69.48	IC50	=	69480	nM
4124.12	IC50	=	4124120	nM	Outside typical range
33.13	IC50	=	33130	nM
19.1	IC50	=	19100	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Solute carrier family 22 member 1

External ID: CHEMBL5229528

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J Nat Prod
Year: 2023
Volume: 86
Issue: 1.0
First Page: 191
Last Page: 198
DOI: 10.1021/acs.jnatprod.2c00927

Target ChEMBL ID: CHEMBL5685
ChEMBL Target Name: Solute carrier family 22 member 1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
38.4	IC50	=	38400	nM
46.96	IC50	=	46960	nM
28	IC50	=	28000	nM
35.18	IC50	=	35180	nM
3.46	IC50	=	3460	nM
40.15	IC50	=	40150	nM
41.28	IC50	=	41280	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089638

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HEK293

External ID: CHEMBL5229525

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2023
Volume: 86
Issue: 1.0
First Page: 191
Last Page: 198
DOI: 10.1021/acs.jnatprod.2c00927

Target ChEMBL ID: CHEMBL614818
ChEMBL Target Name: HEK293
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Solute carrier family 22 member 1

External ID: CHEMBL5229524

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2023
Volume: 86
Issue: 1.0
First Page: 191
Last Page: 198
DOI: 10.1021/acs.jnatprod.2c00927

Target ChEMBL ID: CHEMBL5685
ChEMBL Target Name: Solute carrier family 22 member 1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%
Inhibition	>	50	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HEK293

External ID: CHEMBL5229527

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2023
Volume: 86
Issue: 1.0
First Page: 191
Last Page: 198
DOI: 10.1021/acs.jnatprod.2c00927

Target ChEMBL ID: CHEMBL614818
ChEMBL Target Name: HEK293
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HEK293

External ID: CHEMBL5229526

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2023
Volume: 86
Issue: 1.0
First Page: 191
Last Page: 198
DOI: 10.1021/acs.jnatprod.2c00927

Target ChEMBL ID: CHEMBL614818
ChEMBL Target Name: HEK293
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic
Activity	Non-Toxic

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089633

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089630

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Dose-dependent effect

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type 1G

External ID: CHEMBL3430791

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL5258
ChEMBL Target Name: CaM kinase I gamma
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.9	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	2.7	degrees C
Thermal melting change	=	5.7	degrees C
Thermal melting change	=	7.3	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	1.9	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	-0.1	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type II subunit alpha

External ID: CHEMBL3430792

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4147
ChEMBL Target Name: CaM kinase II alpha
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	4.1	degrees C
Thermal melting change	=	5.7	degrees C
Thermal melting change	=	5	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	2.2	degrees C
Thermal melting change	=	2.2	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	0.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089637

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：The Scripps Research Institute Molecular Screening Center 靶标：N/A

External ID: FBW7_ACT_ALPHA_1536_1X%ACT PRUN

Protocol: Assay Overview:
FBW7 assay principle. In this assay, either mutant or wild type (w.t.) FBW7 interact with phosphorylated cyclin E peptide (cycE~P), which will bring donor and acceptor beads into close proximity. Laser excitation of the donor beads converts oxygen to an excited singlet state. Reaction of the singlet oxygen with the acceptor beads further activates a chemiluminescence/fluorescence reaction within the same bead resulting in emitted light at 520-620 nm. Small molecule activators that enhance the mutant FBW7 interaction with the cycE~P decrease the distance of the acceptor beads, thus leading to increased signal being emitted signal.
Protocol Summary:
There are six steps in this 1536 well assay format which are listed in order. First, 2.5uL/well of a 2X working solution containing RLFbw7 [12.5nM final], Cyclin E peptide [12.5nM final], and Ni beads [5ug/mL final], in assay buffer (25mM Tris-HCl pH 7.4 + 100mM NaCl, 0.1% Tween-20, 5mM ?-Mercaptoethanol and 0.05% BSA) was dispensed into columns 1-44. Then 2.5uL/well of a 2X working solution containing WTFbw7 [12.5nM final], Cyclin E peptide [12.5nM final], and Ni beads [5ug/mL final], in assay buffer was dispensed into columns 45-48. Using the pintool transfer device 134nL of compound or control was added to each well. This achieved a nominal screening concentration of 26.1uM and 2.6% DMSO concentration. This was followed by the addition of SA beads to all wells at 5ug/mL final concentration in assay buffer. The assay was then incubated for 20 hours in a temperature controlled 25C environment followed by Alphascreen detection using the PerkinElmer EnVision.

The percent activation for each compound was calculated as follows:

100 *( ( Test_Compound - Median_Low_Control ) / ( Median_High_Control - Median_Low_Control ) )
Where:
Test_Compound is defined as wells containing RLFbw7 (mutant), cyclin E peptide and Nickel acceptor beads in the presence of test compound
High_Control is defined as wells containing WTFbw7 (wild type), cyclin E peptide and Nickel acceptor beads
Low_Control is defined as the median of the wells containing DMSO, RLFbw7 (mutant), cyclin E peptide and Nickel acceptor beads
PubChem Activity Outcome and Score:

A mathematical algorithm was used to determine active compounds. Two values were calculated: (1) the average percent activation of all compounds tested for the screen, and (2) three times their standard deviation. The sum of these two values was used as a cutoff parameter, i.e. any compound that exhibited greater percent activation than the cutoff parameter (1.85% in the case here) was declared active.
The reported PubChem Activity Score has been normalized to 100% observed primary inhibition. Negative % inhibition values are reported as activity score zero.
The activity score range for active compounds is 100-1, for inactive 1-0.
List of Reagents:
Ni Beads- PerkinEmer Lifesciences Cat#6760619R
RLFbw7-Assay Provider
WTFbw7-Assay Provider
Cyclin E peptide-Assay Provider
5M NaCl- Sigma Cat# S6546-1L
Tween20- Fisher Cat# BP337
Tris 1M pH7.4 Research Organics Cat# 9686T
BSA-Sigma Cat#A7030
?-Mercaptoethanol-SigmaM6250
1536-well plates (Corning, part 7254)

Comment: Due to the size of the Scripps Molecular Screening Center compound library, this assay may have been run as two or more separate campaigns, each campaign testing a unique set of compounds. All data reported were normalized on a per-plate basis. Possible artifacts of this assay can include, but are not limited to: dust or lint located in or on wells of the microtiter plate, compounds that modulate well fluorescence. All test compound concentrations reported above and below are nominal; the specific test concentration(s) for a particular compound may vary based upon the actual sample provided by the Scripps Molecular Screening Center.

Inhibition at 26.1 uM
1.22
1.22
1.22
1.22
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21
1.21

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：5'-AMP-activated protein kinase catalytic subunit alpha-2

External ID: CHEMBL3430789

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2116
ChEMBL Target Name: AMP-activated protein kinase, alpha-2 subunit
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.9	degrees C
Thermal melting change	=	1.5	degrees C
Thermal melting change	=	-1	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	7	degrees C
Thermal melting change	=	7.4	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	-0.7	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	6.4	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	-0.9	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Calcium/calmodulin-dependent protein kinase type 1D

External ID: CHEMBL3430790

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL5073
ChEMBL Target Name: CaM kinase I delta
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	2.9	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	3.6	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.1	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL2089635

Protocol: N/A

Comment: Journal: Eur. J. Med. Chem.
Year: 2012
Volume: 56
First Page: 361
Last Page: 367
DOI: 10.1016/j.ejmech.2012.07.041

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Serine/threonine-protein kinase pim-1

External ID: CHEMBL3430827

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2147
ChEMBL Target Name: Serine/threonine-protein kinase PIM1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-2.1	degrees C
Thermal melting change	=	3.7	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	5.9	degrees C
Thermal melting change	=	7	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	8.1	degrees C
Thermal melting change	=	2.9	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-1	degrees C
Thermal melting change	=	-1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-1.4	degrees C
Thermal melting change	=	-1.2	degrees C
Thermal melting change	=	4.8	degrees C
Thermal melting change	=	3.7	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	-0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Serine/threonine-protein kinase pim-2

External ID: CHEMBL3430828

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4523
ChEMBL Target Name: Serine/threonine-protein kinase PIM2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	3.8	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	4	degrees C
Thermal melting change	=	5.2	degrees C
Thermal melting change	=	3.1	degrees C
Thermal melting change	=	8.6	degrees C
Thermal melting change	=	2.3	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	7.1	degrees C
Thermal melting change	=	5.1	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Cyclin-dependent kinase 16

External ID: CHEMBL3430825

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4597
ChEMBL Target Name: Serine/threonine-protein kinase PCTAIRE-1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.8	degrees C
Thermal melting change	=	1.2	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	1.7	degrees C
Thermal melting change	=	3	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	3.5	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	-0.6	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	-0.9	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-0.4	degrees C
Thermal melting change	=	8.1	degrees C
Thermal melting change	=	-0.4	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：3-phosphoinositide-dependent protein kinase 1

External ID: CHEMBL3430826

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2534
ChEMBL Target Name: 3-phosphoinositide dependent protein kinase-1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-1.9	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	2.5	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	-0.7	degrees C
Thermal melting change	=	2.9	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-1	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	8.6	degrees C
Thermal melting change	=	-1.3	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	0	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Serine/threonine-protein kinase PLK4

External ID: CHEMBL3430831

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL3788
ChEMBL Target Name: Serine/threonine-protein kinase PLK4
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	5.2	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	4.2	degrees C
Thermal melting change	=	3	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	7.4	degrees C
Thermal melting change	=	7.8	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	3.3	degrees C
Thermal melting change	=	11.8	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	6.2	degrees C
Thermal melting change	=	-0.2	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：cAMP-dependent protein kinase catalytic subunit alpha

External ID: CHEMBL3430832

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4101
ChEMBL Target Name: cAMP-dependent protein kinase alpha-catalytic subunit
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	1.7	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	3.6	degrees C
Thermal melting change	=	2.1	degrees C
Thermal melting change	=	4.2	degrees C
Thermal melting change	=	2	degrees C
Thermal melting change	=	9.2	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	5.9	degrees C
Thermal melting change	=	2.6	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	6.5	degrees C
Thermal melting change	=	0.4	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Serine/threonine-protein kinase pim-3

External ID: CHEMBL3430829

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL5407
ChEMBL Target Name: Serine/threonine-protein kinase PIM3
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0	degrees C
Thermal melting change	=	4.6	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	6.3	degrees C
Thermal melting change	=	9.1	degrees C
Thermal melting change	=	3	degrees C
Thermal melting change	=	8.8	degrees C
Thermal melting change	=	4.7	degrees C
Thermal melting change	=	7.1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	1.3	degrees C
Thermal melting change	=	7.2	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	6.7	degrees C
Thermal melting change	=	0.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Serine/threonine-protein kinase PLK1

External ID: CHEMBL3430830

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL3024
ChEMBL Target Name: Serine/threonine-protein kinase PLK1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	2	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	0.6	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	1.7	degrees C
Thermal melting change	=	0.5	degrees C
Thermal melting change	=	1.5	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-0.9	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	1.6	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	-1.4	degrees C
Thermal melting change	=	3.9	degrees C
Thermal melting change	=	11.9	degrees C
Thermal melting change	=	0.1	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	1.3	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Ribosomal protein S6 kinase alpha-3

External ID: CHEMBL3430835

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL2345
ChEMBL Target Name: Ribosomal protein S6 kinase alpha 3
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	1	degrees C
Thermal melting change	=	0.8	degrees C
Thermal melting change	=	1.5	degrees C
Thermal melting change	=	6.6	degrees C
Thermal melting change	=	6.8	degrees C
Thermal melting change	=	8.6	degrees C
Thermal melting change	=	-0.1	degrees C
Thermal melting change	=	-0.2	degrees C
Thermal melting change	=	1.4	degrees C
Thermal melting change	=	6.3	degrees C
Thermal melting change	=	0.3	degrees C
Thermal melting change	=	0.7	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	7.5	degrees C
Thermal melting change	=	-0.3	degrees C
Thermal melting change	=	3.4	degrees C
Thermal melting change	=	-0.4	degrees C

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：STE20-like serine/threonine-protein kinase

External ID: CHEMBL3430836

Protocol: N/A

Comment: Journal: Proc Natl Acad Sci U S A
Year: 2007
Volume: 104
Issue: 51
First Page: 20523
Last Page: 20528
DOI: 10.1073/pnas.0708800104

Target ChEMBL ID: CHEMBL4202
ChEMBL Target Name: Serine/threonine-protein kinase 2
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Thermal melting change	=	3.9	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	0.4	degrees C
Thermal melting change	=	4.6	degrees C
Thermal melting change	=	6.8	degrees C
Thermal melting change	=	2.4	degrees C
Thermal melting change	=	5.7	degrees C
Thermal melting change	=	7.5	degrees C
Thermal melting change	=	6.5	degrees C
Thermal melting change	=	1.1	degrees C
Thermal melting change	=	0	degrees C
Thermal melting change	=	0.2	degrees C
Thermal melting change	=	8.2	degrees C
Thermal melting change	=	4.4	degrees C
Thermal melting change	=	5.4	degrees C
Thermal melting change	=	4.1	degrees C
Thermal melting change	=	1.8	degrees C
Thermal melting change	=	1.7	degrees C
Thermal melting change	=	3.5	degrees C
Thermal melting change	=	0.4	degrees C

524-12-9 靶点实验数据