Sensitive and rapid electrochemical bioassay of glycosidase activity.

Tanin Tangkuaram, Jared Q Gerlach, Yun Xiang, Abdel-Nasser Kawde, Zong Dai, Veer P Bhavanandan, Jeffrey T La Belle, Waret Veerasai, Lokesh Joshi, Joseph Wang

Index: Analyst 131(8) , 889-91, (2006)

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Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.