Pressure induced structural fluctuations in hemoglobin, studied by EPR-spectroscopy.

J Paul, E von Goldammer, H R Wenzel

Index: Z. Naturforsch., C, J. Biosci. 43(3-4) , 162-6, (1988)

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Abstract

A quartz based cavity for pressure dependent EPR measurements on liquid samples allowing pressures up to 0.6 GPa was constructed. First investigations with this setup were done on spin labeled horse hemoglobin derivatives both in ferric and ferrous state of oxidation. The second derivative EPR spectra show changes of the label's mobility, which are not correlated with spin state changes of the Fe-porphyrin complex, but which point out structural fluctuations inside the globin protein matrices.