Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus.

Stefan Söllner, Silvia Berkner, Georg Lipps

Index: Extremophiles 10(6) , 629-34, (2006)

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Abstract

A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography. The enzyme cuts DNA at the recognition site GCwGC as could be shown by restriction analysis of plasmids and short synthetic duplex DNA. The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C.