最佳答案
回答者：网友
1. Dilute your primary antibody in your blocking buffer
2. Incubate 实江裂your coversl换双资院系血按青航ips with primary antibody for 1 hours at room temperature or 4 degrees ove司离治集妈院纪施初rnight
3. 九溶交合坐临广燃周wash 3x PBS
4.your sample is ready for secon容全dary antibody
