产品货号:jlcR3611
英文名称:EEF2
中文名称:真核翻译延长因子2抗体
别名:EEF2; EF2; Elongation factor 2; Eukaryotic translation elongation factor 2; Eukaryotic Elongation Factor-2; EF2_HUMAN; EF-2. .
研究领域:肿瘤细胞生物免疫学信号转导转录调节因子
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human, Mouse, Rat, Chicken, Pig, Cow, Horse,
产品应用:WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分子量:94kDa
细胞定位:细胞浆
性状:Lyophilized or Liquid
浓度: 1mg/ml
免疫原:KLH conjugated synthetic peptide derived from human EEF2:521-620/858
亚型:IgG
纯化方法:affinity purified by Protein A
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed :PubMed
产品介绍: EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Function:
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Subunit:
Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.
Subcellular Location:
Cytoplasm.
Post-translational modifications:
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.
Similarity:
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
SWISS:
P13639
Gene ID:
1938
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
